Human IL-1 beta /IL-1F2 Propeptide Antibody Summary
Met1-Asp116
Accession # P01584
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human IL‑1 beta /IL‑1F2 Propeptide by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 80 nM PMA for 48 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL-1 beta /IL-1F2 Propeptide Monoclonal Antibody (Catalog # MAB6964) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-1 beta /IL-1F2 Propeptide at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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IL‑1 beta /IL‑1F2 in Human PBMCs. IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells using Mouse Anti-Human IL-1 beta /IL-1F2 Propeptide Monoclonal Antibody (Catalog # MAB6964) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI(blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Detection of Human IL‑1 beta /IL‑1F2 Propeptide by Simple WesternTM. Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with PMA and LPS, loaded at 0.2 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 Propeptide at approximately 40 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IL‑1 beta /IL‑1F2 Propeptide Monoclonal Antibody (Catalog # MAB6964). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2
IL-1 beta, also known as IL1F2, is a prototypical member of the IL-1 superfamily. It is produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. The biological activity of IL-1 beta is mediated by the heterodimeric receptor complex containing IL-1 R1 (IL-1 RI) and IL-1 R3 (IL-1 RAcP). The IL-1 beta propeptide is cleaved intracellularly by caspase-1/ICE to generate a 17 kDa active cytokine. The propeptide of human IL-1 beta shares approximately 55% aa sequence identity with the propeptides of mouse and rat IL-1 beta.
Product Datasheets
Citations for Human IL-1 beta /IL-1F2 Propeptide Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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An IL-1?-driven neutrophil-stromal cell axis fosters a BAFF-rich protumor microenvironment in individuals with multiple myeloma
Authors: de Jong, MME;Fokkema, C;Papazian, N;Czeti, Á;Appelman, MK;Vermeulen, M;van Heusden, T;Hoogenboezem, RM;van Beek, G;Tahri, S;Sanders, MA;van de Woestijne, PC;Gay, F;Moreau, P;Büttner-Herold, M;Bruns, H;van Duin, M;Broijl, A;Sonneveld, P;Cupedo, T;
Nature immunology
Species: Human
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Levistilide A Ameliorates NLRP3 Expression Involving the Syk-p38/JNK Pathway and Peripheral Obliterans in Rats
Authors: H Guo, L Sun, S Ling, JW Xu
Mediators Inflamm., 2018-08-12;2018(0):7304096.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Azithromycin decreases NALP3 mRNA stability in monocytes to limit inflammasome-dependent inflammation
Authors: EA Lendermon, TA Coon, JS Bednash, NM Weathingto, JF McDyer, RK Mallampall
Respir. Res., 2017-06-28;18(1):131.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
MicroRNA-9 Inhibits NLRP3 Inflammasome Activation in Human Atherosclerosis Inflammation Cell Models through the JAK1/STAT Signaling Pathway
Authors: Y Wang, Z Han, Y Fan, J Zhang, K Chen, L Gao, H Zeng, J Cao, C Wang
Cell. Physiol. Biochem, 2017-03-27;41(4):1555-1571.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
NLRP3 inflammasome: a novel link between lipoproteins and atherosclerosis
Arch Med Sci, 2016-07-20;12(5):950-958.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
NLRP3 and downstream cytokine expression elevated in the monocytes of patients with coronary artery disease
Authors: Lili Wang, Peng Qu, Jian Zhao, Yeting Chang
Archives of Medical Science
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Unsaturated fatty acids prevent activation of NLRP3 inflammasome in human monocytes/macrophages.
Authors: L'homme L, Esser N, Riva L, Scheen A, Paquot N, Piette J, Legrand-Poels S
J Lipid Res, 2013-09-03;54(11):2998-3008.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Propionibacterium acnes Induces IL-1beta secretion via the NLRP3 inflammasome in human monocytes.
Authors: Qin M, Pirouz A, Kim M, Krutzik S, Garban H, Kim J
J Invest Dermatol, 2013-07-24;134(2):381-8.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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