Human Importin alpha 2/KPNA2 Antibody (682208)

R&D Systems | Catalog # MAB62071

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 682208
View all Importin alpha 2/KPNA2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Importin alpha 2/KPNA2
Ser2-Asn132
Accession # P52292

Specificity

Detects human Importin alpha 2/KPNA2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) KPNA1, rhKPNA3, rhKPNA4, rhKPNA5, or rhKPNB1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Importin alpha 2/KPNA2 Antibody (682208)

Importin a2/KPNA2 antibody in NTera-2 Human Cell Line by Immunocytochemistry (ICC).

Importin alpha 2/KPNA2 in NTera‑2 Human Cell Line.

Importin a2/KPNA2 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Mouse Anti-Human Importin a2/KPNA2 Monoclonal Antibody (Catalog # MAB62071) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Importin  alpha 2/KPNA2 in HeLa Human Cell Line.

Importin  alpha 2/KPNA2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Importin  alpha 2/KPNA2 Monoclonal Antibody (Catalog # MAB62071) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Importin alpha 2/KPNA2 Antibody (682208)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed NTera‑2 human testicular embryonic carcinoma cell line and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Importin alpha 2/KPNA2

Importin subunit alpha 2, also known as KPNA2 (Karyopherin subunit alpha 2), SRP1a, and RAG1, is a 58-60 kDa member of the Importin alpha family of proteins. It is ubiquitously expressed, and found in both nucleus and cytoplasm. Importin alpha 2 functions as a cargo carrier that transports various complexes from cytoplasm into nucleus. For the MRN DNA repair complex, Importin alpha 2 first binds to NBS1, and then to Importin beta 1, which mediates movement through the nuclear pore. Human Importin alpha 2 is 529 amino acids (aa) in length. It contains an N-terminal IBB/Importin b domain (aa 2-60), ten Armadillo repeats that bind “cargo” (aa 71-496) and two intervening NLS binding sites. There is one splice variant that shows a deletion of aa 72-310, coupled to an 11 aa substitution for aa 389-406. The aminoacid region 1-132 of human Importin alpha 2 shares a 94% homology with mouse Importin alpha 2.

Long Name

Karyopherin alpha 2

Alternate Names

Importin alpha 1, IPOA1, KPNA2, Pendulin, QIP2, RCH1, SRP1-alpha

Entrez Gene IDs

3838 (Human); 16647 (Mouse); 85245 (Rat)

Gene Symbol

KPNA2

UniProt

P52292 (Human)

Additional Importin alpha 2/KPNA2 Products

Product Documents for Human Importin alpha 2/KPNA2 Antibody (682208)

Certificate of Analysis

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Product Specific Notices for Human Importin alpha 2/KPNA2 Antibody (682208)

For research use only

Related Research Areas

Cancer Biomarkers
Cell Cycle

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