KPNA2 (Karyopherin subunit alpha 2; also SRP1 alpha, importin subunit alpha 2 and RAG1) is a 58-60 kDa member of the importin alpha family of proteins. It is ubiquitously expressed, and found in both nucleus and cytoplasm. KPNA2 functions as a cargo carrier that transports various complexes from cytoplasm into nucleus. For the MRN DNA repair complex, KPNA2 first binds to NBS1, and then to importin beta 1, which mediates movement through the nuclear pore. Human KPNA2 is 529 amino acids (aa) in length. It contains an N-terminal IBB/importin beta domain (aa 2-60), ten Armadillo repeats that bind "cargo" (aa 71-496) and three intervening NLSs. There is one splice variant that shows a deletion of aa 72-310, coupled to an 11 aa substitution for aa 389-406. Over aa 1-132, human KPNA2 shares 94% aa identity with mouse KPNA2.
Human Importin alpha 2/KPNA2 Antibody
R&D Systems | Catalog # AF6207
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human Importin alpha 2/KPNA2
Met1-Asp132
Accession # P52292
Met1-Asp132
Accession # P52292
Specificity
Detects human Importin alpha 2/KPNA2 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Importin alpha 2/KPNA2 Antibody
Detection of Human Importin alpha 2/KPNA2 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF-7 human breast cancer cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human Importin a2/KPNA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6207) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Importin a2/KPNA2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Importin alpha 2/KPNA2 in NTera‑2 Human Cell Line.
Importin a2/KPNA2 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Sheep Anti-Human Importin a2/KPNA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6207) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Importin alpha 2/KPNA2 in HeLa Human Cell Line.
Importin alpha 2/KPNA2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Importin alpha 2/KPNA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6207) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human Importin alpha 2/KPNA2 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed NTera‑2 human testicular embryonic carcinoma cell line and HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed NTera‑2 human testicular embryonic carcinoma cell line and HeLa human cervical epithelial carcinoma cell line
Western Blot
0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, MCF‑7 human breast cancer cell line, and HeLa human cervical epithelial carcinoma cell line
Sample: Jurkat human acute T cell leukemia cell line, MCF‑7 human breast cancer cell line, and HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Importin alpha 2/KPNA2
Long Name
Karyopherin alpha 2
Alternate Names
Importin alpha 1, IPOA1, KPNA2, Pendulin, QIP2, RCH1, SRP1-alpha
Gene Symbol
KPNA2
UniProt
Additional Importin alpha 2/KPNA2 Products
Product Documents for Human Importin alpha 2/KPNA2 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Importin alpha 2/KPNA2 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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