Human Kallikrein 3/PSA Antibody (181811)
R&D Systems | Catalog # MAB13442
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Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Pro19-Pro261
Accession # P07288
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Kallikrein 3/PSA Antibody (181811)
Detection of Human Kallikrein 3/PSA by Western Blot.
Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Kallikrein 3/PSA Monoclonal Antibody (Catalog # MAB13442) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Kallikrein 3/PSA at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Human Kallikrein 3 / PSA ELISA Standard Curve
Recombinant Human Kallikrein 3/PSA (Catalog # 1344-SE) was serially diluted and captured by Mouse Anti-Human Kallikrein 3/PSA Monoclonal Antibody (Catalog # MAB13442) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Kallikrein 3/PSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1344) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human Kallikrein 3/PSA Antibody (181811)
Western Blot
Sample: Human prostate tissue
Human Kallikrein 3/PSA Sandwich Immunoassay
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Kallikrein 3/PSA
Additional Kallikrein 3/PSA Products
Product Documents for Human Kallikrein 3/PSA Antibody (181811)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Kallikrein 3/PSA Antibody (181811)
For research use only
Citations for Human Kallikrein 3/PSA Antibody (181811)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars