Solute Carrier, family 12 (potassium/chloride transporter), member 5 (SLC12A5) encodes KCC2, a multi-pass co-transporter for K+ and Cl-. KCC2/SLC12A5 is expressed on neurons, and is dysregulated in schizophrenia, spasticity, and affective disorders.
Human KCC2/SLC12A5 Antibody
R&D Systems | Catalog # MAB8369
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 905705
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Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human SLC12A5
Accession # Q9H2X9
Accession # Q9H2X9
Specificity
Detects human SLC12A5 in ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human KCC2/SLC12A5 Antibody
Detection of Human KCC2/SLC12A5 by Western Blot.
Western blot shows lysates of human brain stem tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human KCC2/SLC12A5 Monoclonal Antibody (Catalog # MAB8369) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for KCC2/SLC12A5 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of KCC2/SLC12A5 in HEK293 Human Cell Line Transfected with Human KCC2/SLC12A5 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with human KCC2/SLC12A5 and eGFP was stained with either (A) Mouse Anti-Human KCC2/SLC12A5 Monoclonal Antibody (Catalog # MAB8369) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Applications for Human KCC2/SLC12A5 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human KCC2/SLC12A5 and eGFP
Sample: HEK293 human embryonic kidney cell line transfected with human KCC2/SLC12A5 and eGFP
Western Blot
0.5 µg/mL
Sample: Human brain stem tissue
Sample: Human brain stem tissue
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: KCC2/SLC12A5
Long Name
K-Cl Cotransporter 2
Alternate Names
SLC12A5
Gene Symbol
SLC12A5
UniProt
Additional KCC2/SLC12A5 Products
Product Documents for Human KCC2/SLC12A5 Antibody
Product Specific Notices for Human KCC2/SLC12A5 Antibody
For research use only
Related Research Areas
Citations for Human KCC2/SLC12A5 Antibody
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