Human KIR3DL2/CD158k Alexa Fluor™ Plus 488‑conjugated Antibody

R&D Systems | Catalog # FAB2878AFP488

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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 539304
View all KIR3DL2/CD158k Primary Antibodies »

Product Specifications

Specificity

Detects human KIR3DL2/CD158k in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human KIR3DL2/CD158k Alexa Fluor™ Plus 488‑conjugated Antibody

Application
Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: KIR3DL2/CD158k

KIR3DL2 (3DL2, p140, CD158k) is a type I transmembrane protein of the p70 family of killer cell Ig-like receptors (KIR). KIR are expressed on CD56dim NK cells and T cell subsets where they participate in identifying normal and abnormal cells and regulating effector functions of the innate immune system (1-4). KIR are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD) and whether they have long or short (L, S) cytoplasmic tails. Like other inhibitory KIR, KIR3DL2 has two ITIM domains within its long tail (3). KIR3DL2 is diverse, with twelve alleles identified and as many as five single amino acid polymorphisms found in a single individual (4-6). Unlike most other KIR, gene transcripts of KIR3DL2 are expressed by all individuals (4). KIR3DL2 is present on the cell surface as a disulfide-linked homodimer of 70 kDa, 434 aa subunits (4). KIR3DL2 has shown peptide-specific binding to some HLA-A antigens, including A3 and A11 (4, 7, 8).  It also binds the abnormally folded HLA-B27 homodimer found in spondylarthritides, but not the normal heterodimer of HLA-B27 with beta 2‑microglobulin (9, 10). NK and CD4+ T cells from patients with spondylarthritides show increased KIR3DL2+ expression and may play a role in disease pathology (10). KIR3DL2 is also a marker for atypical mononuclear (Sezary) cells in the blood of patients with Sezary syndrome, an erythrodermic form of cutaneous T cell lymphoma (11). Human KIR3DL2 ECD shows 88-92% aa identity to KIR3DL2 of other primates. KIR receptors have no structural orthologs in non-primates, although mouse Ly-49 proteins are functional orthologs (3). KIR are highly related. The closest relative, KIR3DL1 shows 86% aa identity with KIR3DL2 within the ECD.

References

  1. Colonna, M. and J. Samaridis (1995) Science 268:405.
  2. Pende, D. et al. (1996) J. Exp. Med. 184:505.
  3. Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
  4. Uhrberg, M. et al. (1997) Immunity 7:753.
  5. Meenaugh, A. et al. (2004) Tiss. Antigens 64:226.
  6. Kwon, D. et al. (2000) Mol. Cells 10:54.
  7. Dohring, C. et al. (1996) J. Immunol. 156:3098.
  8. Hansasuta, P. et al. (2004) Eur. J. Immunol. 34:1673.
  9. Kollnberger, S. et al. (2002) Arthritis Rheum. 46:2972.
  10. Chan, A. T. et al. (2005) Arthritis Rheum. 52:3586.
  11. Poszepczynska-Guigne, E. et al. (2004) J. Invest. Dermatol. 122:820.

Long Name

Killer Cell Immunoglobulin-like Receptor, Three Domain Long Cytoplasmic Tail, 2

Alternate Names

CD158k, CL-5, NKAT4A, NKAT4B

Entrez Gene IDs

3812 (Human); 245615 (Mouse)

Gene Symbol

KIR3DL2

UniProt

P43630

Additional KIR3DL2/CD158k Products

Product Documents for Human KIR3DL2/CD158k Alexa Fluor™ Plus 488‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human KIR3DL2/CD158k Alexa Fluor™ Plus 488‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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