Human Klotho beta Antibody (885935)

R&D Systems | Catalog # MAB5889

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot, ELISA Development (Detection)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 885935
View all Klotho beta Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Klotho beta
Phe53-Leu997
Accession # Q86Z14

Specificity

Detects human Klotho beta in direct ELISA and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Klotho beta Antibody (885935)

Detection of Human Klotho beta antibody by Western Blot.

Detection of Human Klotho beta by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Klotho beta Monoclonal Antibody (Catalog # MAB5889) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Klotho beta at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Klotho beta antibody by Simple WesternTM.

Detection of Human Klotho beta by Simple WesternTM.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Klotho beta at approximately 177/170 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Klotho beta Monoclonal Antibody (Catalog # MAB5889). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Klotho beta Antibody (885935)

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Klotho beta

Klotho beta (b-Klotho or KLB) is a 125-135 kDa member of the KL family, Glycosyl Hydrolase 1 superfamily of proteins.  It plays key role in the biology of the endocrine FGFs (FGF-15/19; 21; 23).  KLB appears to serve as a signaling cofactor for select FGFRs, including FGFR-1c, -2c, -3c and FGFR4.  The cofactor is known to constitutively interact with FGFRs, promote FGF binding to their appropriate FGFRs, and to bind directly to FGF-19 and FGF-21.  Notably, FGF-23 appears to utilize a-Klotho/KL for its FGFR signaling cofactor, and FGF-1, while not dependent upon KLB for signaling, can utilize KBL to potentiate certain activities.  KLB is expressed on select cells, including adipocytes and hepatocytes.  Human KLB is a 1044 amino acids (aa) type III transmembrane (TM) protein (i.e.-a type I TM protein lacking a signal sequence).  It contains a 996 aa extracellular region (aa 1-996) and a 27 aa cytoplasmic domain (aa 1018-1044).  The extracellular region contains two glycosyl hydrolase domains (aa 77-508 and 517-967) plus eleven potential N-linked glycosylation sites. Neither hydrolase domain is active as they lack a key Glu residue needed for activity.  Over aa 53-997, human KLB shares 80% aa sequence identity with mouse KLB.  And relative to KL/a-Klotho, human KLB and KL share just 44% aa sequence identity over the entire length of the molecules.     

Alternate Names

KLB

Entrez Gene IDs

152831 (Human); 83379 (Mouse); 289625 (Rat); 102147155 (Cynomolgus Monkey)

Gene Symbol

KLB

UniProt

Q86Z14

Additional Klotho beta Products

Product Documents for Human Klotho beta Antibody (885935)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Klotho beta Antibody (885935)

For research use only

Citations for Human Klotho beta Antibody (885935)

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