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Human Langerin/CD207 Antibody

Catalog #: MAB2088
3 Citations Datasheet / COA / SDS

Discontinued Product

MAB2088 has been discontinued.
View all Langerin/CD207 products.
Detection of Langerin/CD207 in Human Immature Dentritic Cells by Flow Cytometry.
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Citations (3)
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Human Langerin/CD207 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Langerin/CD207 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 343828
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Langerin/CD207
Tyr64-Pro328
Accession # Q9UJ71
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Langerin/CD207 (Catalog # 2088-LN)
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Langerin/CD207 antibody in Human Immature Dentritic Cells antibody by Flow Cytometry. View Larger

Detection of Langerin/CD207 in Human Immature Dentritic Cells by Flow Cytometry. Human immature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14+cells were treated with 20 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) and 50 ng/mL Recombinant Human GM-CSF (Catalog # 215-GM) for 6-7 days and then stained with Mouse Anti-Human Langerin/CD207 Monoclonal Antibody (Catalog # MAB2088, filled histogram) or isotype control (Catalog # MAB002, open histogram), followed by Goat F(ab)2 Anti-Mouse IgG (H+L) Allophycocyanin (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Langerin/CD207

Langerin (also known as CD207) is a type II transmembrane glycoprotein which is member K of the C-type lectin domain family 4 (1). Langerin is used as a marker for Langerhans cells (LCs) which represent the immature dendritic cells in the epidermis (1, 2). LCs uniquely contain “tennis racket”-shaped endosomal recycling compartment subdomains with pentalamellar membranes termed Birbeck granules (1‑3). Langerin is necessary and sufficient for Birbeck granule formation (1). The 328 amino acid (aa) human langerin sequence contains a 43 aa cytoplasmic domain, a 21 aa transmembrane domain and a 264 aa extracellular domain (ECD) that contains a coiled-coil domain and a single C-type lectin domain. Trimerization greatly increases the lectin binding affinity (4). Langerin internalizes endogenous proteins such as type I procollagen. Internalization by LC is thought to lead to suppression of self reactions (4‑6). Langerin also mediates endocytosis of non‑peptide antigens containing mannose, N-acetyl glucosamine and fucose that are expressed by mycobacteria and fungae (4, 7). Some antigens, such as the M. leprae glycolipid arabinomycolate, are ultimately presented by human LC CD1a in cutaneous-draining lymph nodes (8). Langerin performs a barrier-like function to HIV-1 transmission due to its internalization of virus particles for destruction (9). A rare human polymorphism within the lectin domain, W264R, abolishes both carbohydrate recognition and Birbeck granule formation (10, 11). Genetic deletion of mouse langerin was not shown to have functional consequence other than abolishing Birbeck granule formation (12). Human langerin shares 68%, 62%, 71% aa identity with mouse, rat and bovine langerin ECD, respectively.

References
  1. Valladeau, J. et al. (2000) Immunity 12:71. 
  2. Valladeau, J. et al. (2003) Immunol. Res. 28:93. 
  3. McDermott, R. et al. (2002) Mol. Biol. Cell 13:317. 
  4. Stambach, N. S. and M. E. Taylor (2003) Glycobiology 13:401. 
  5. Tada, Y. et al. (2006) J. Invest. Dermatol. 126:1549. 
  6. Ritter, U. and A. Osterloh (2007) Med. Microbiol. Immunol. 196:51. 
  7. Takahara, K. et al. (2003) Int. Immunol. 16:819.
  8. Hunger, R. E. et al. (2004) J. Clin. Invest. 113:701.
  9. De Witte, L. et al. (2007) Nat. Med. 13:367.
  10. Verdijk, P. et al. (2005) J. Invest. Dermatol. 124:714.
  11. Ward, E. M. et al. (2006) J. Biol. Chem. 281:15450.
  12. Kissenpfennig, A. et al. (2005) Mol. Cell. Biol. 25:88.
Entrez Gene IDs
50489 (Human); 246278 (Mouse)
Alternate Names
CD207 antigen; CD207 antigen, langerin; CD207 molecule, langerin; CD207; CLEC4KLangerhans cell specific c-type lectin; C-type lectin domain family 4 member K; C-type lectin domain family 4, member K; Langerin

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Citations for Human Langerin/CD207 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Notch is active in Langerhans cell histiocytosis and confers pathognomonic features on dendritic cells.
    Authors: Hutter, Caroline, Kauer, Max, Simonitsch-Klupp, Ingrid, Jug, Gunhild, Schwentner, Raphaela, Leitner, Judith, Bock, Peter, Steinberger, Peter, Bauer, Wolfgang, Carlesso, Nadia, Minkov, Milen, Gadner, Helmut, Stingl, Georg, Kovar, Heinrich, Kriehuber, Ernst
    Blood, 2012-10-16;120(26):5199-208.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. HIV-1 replication in Langerhans and interstitial dendritic cells is inhibited by neutralizing and Fc-mediated inhibitory antibodies.
    Authors: Peressin M, Holl V, Schmidt S, Decoville T, Mirisky D, Lederle A, Delaporte M, Xu K, Aubertin AM, Moog C
    J. Virol., 2010-11-17;85(2):1077-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Generation of mature human myelomonocytic cells through expansion and differentiation of pluripotent stem cell-derived lin-CD34+CD43+CD45+ progenitors.
    Authors: Choi KD, Vodyanik MA, Slukvin II
    J. Clin. Invest., 2009-08-10;119(9):2818-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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