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Human LAP (TGF-beta 1) Antibody

Catalog #: MAB246
13 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB246-SP
MAB246-500
MAB246-100

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LAP (TGF-beta 1) in HEK293 Human Cell Line.
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Citations (13)
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Human LAP (TGF-beta 1) Antibody Summary

Species Reactivity
Human
Specificity
Detects human LAP in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 27235
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP (TGF-beta 1)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
3-25 µg/mL
See below
Neutralization
Measured by its ability to neutralize LAP TGF‑ beta 1 inhibition of TGF‑ beta 1 growth inhibition in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 500 ng/mL Recombinant Human LAP TGF‑ beta 1 and 1 ng/mL Recombinant Human TGF‑ beta 1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry LAP (TGF-beta 1) antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC). View Larger

LAP (TGF-beta 1) in HEK293 Human Cell Line. LAP (TGF-beta 1) was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB246) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Neutralization LAP TGF‑ beta 1 Inhibition of TGF‑ beta 1 Activity and Neutralization by Human LAP TGF‑ beta 1 Antibody. View Larger

LAP TGF‑ beta 1 Inhibition of TGF‑ beta 1 Activity and Neutralization by Human LAP TGF‑ beta 1 Antibody. Recombinant Human LAP TGF-beta 1 (Catalog # 246-LP) inhibits Recombinant Human TGF-beta 1 (Catalog # 240-B) growth inhibition activity in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human TGF-beta 1 (1 ng/mL) activity elicited by Recombinant Human LAP TGF-beta 1 (500 ng/mL) is neutralized (green line) by increasing concentrations of Human LAP TGF-beta 1 Monoclonal Antibody (Catalog # MAB246). The ND50 is typically 1-5 µg/mL.

Western Blot Detection of LAP (TGF-beta 1) by Western Blot View Larger

Detection of LAP (TGF-beta 1) by Western Blot Specificity of R58 and L59 antibodies. (a) Schematic structure of a GST- rhLTGF-beta 1 protein generated in bacteria and a commercial rhLAP beta 1 generated in insect cells. The PLK cleavage site is indicated by arrowhead. (b-e) GST-LTGF-beta 1/rhLAP beta 1was incubated with/without PLK or PLN for 45 min at 37°C, and thereafter equal amounts of samples containing either 50 ng GST-rhLTGF-beta 1 or 500 ng rhLAP beta 1 were subjected to each lane in SDS-PAGE under reducing conditions followed by Western blot analyses using R58/L59 antibodies (b, d, respectively). Membranes used in panels b and d were reprobed with monoclonal (c) or polyclonal (e) anti-LAP antibodies. Lanes 1, intact GST-rhLTGF-beta 1 or rhLAP-beta 1; lanes 2, PLK digested GST-rhLTGF-beta 1 or rhLAP-beta 1; lanes 3, PLK; lane 4, PLN digested GST-rhLTGF-beta 1 or rhLAP-beta 1; lane 5, PLN. Representative results from three independent experiments with a similar result are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24877031), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAP (TGF-beta 1)

The TGF-beta family includes several related proteins (70-80% sequence homology) from mammalian, avian, or Xenopus systems that are now designated TGF-beta 1, TGF‑ beta 2, TGF-beta 1.2, TGF-beta 3, TGF-beta 4, and TGF-beta 5. These proteins are secreted by cells in the form of an inactive complex, referred to as latent TGF-beta, that consists of TGF-beta associated non-covalently with a Latency-associated peptide (LAP). These two proteins are synthesized as a single pro-peptide that is cleaved in a post Golgi compartment prior to secretion. Different TGF-beta family members are naturally associated with their own distinct LAPs.

Long Name
Latency-associated Peptide
Entrez Gene IDs
7040 (Human)
Alternate Names
CED; DPD1; LAP (TGFbeta 1); LAP (TGF-beta 1); LAP; TGFB; TGFB1; TGFbeta; transforming growth factor beta 1

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Citations for Human LAP (TGF-beta 1) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Targeting hypoxia-induced tumor stemness by activating pathogen-induced stem cell niche defense.
    Authors: Bhuyan S, Pal B, Pathak L, Saikia P, Mitra S, Gayan S, Mokhtari R, Li H, Ramana C, Baishya D, Das B
    Front Immunol, 2022-09-29;13(0):933329.
    Species: Complex Species Category
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Chemokine Receptor Expression on T Cells Is Modulated by CAFs and Chemokines Affect the Spatial Distribution of T Cells in Pancreatic Tumors
    Authors: L Gorchs, M Oosthoek, T Yucel-Lind, CF Moro, H Kaipe
    Cancers, 2022-08-06;14(15):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Mechanical stress determines the configuration of TGF&beta activation in articular cartilage
    Authors: G Zhen, Q Guo, Y Li, C Wu, S Zhu, R Wang, XE Guo, BC Kim, J Huang, Y Hu, Y Dan, M Wan, T Ha, S An, X Cao
    Nature Communications, 2021-03-17;12(1):1706.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. The vitamin D analogue calcipotriol promotes an anti-tumorigenic phenotype of human pancreatic CAFs but reduces T cell mediated immunity
    Authors: L Gorchs, S Ahmed, C Mayer, A Knauf, C Fernández, M Svensson, R Heuchel, E Rangelova, P Bergman, H Kaipe
    Sci Rep, 2020-10-15;10(1):17444.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Macrophage-derived HIV-1 carries bioactive TGF-beta
    Authors: A Arakelyan, JD Petersen, J Blazkova, L Margolis
    Sci Rep, 2019-12-13;9(1):19100.
    Species: Virus - HIV-1
    Sample Types: Virus
    Applications: Multiplex Assay
  6. Human Pancreatic Carcinoma-Associated Fibroblasts Promote Expression of Co-inhibitory Markers on CD4+ and CD8+ T-Cells
    Authors: Laia Gorchs, Carlos Fernández Moro, Peter Bankhead, Katharina P. Kern, Imrul Sadeak, Qingda Meng et al.
    Frontiers in Immunology
    Species: Human
    Sample Types: Whoile Cells
    Applications: Neutralization
  7. Reduced CD160 expression contributes to impaired NK cell function and poor clinical outcomes in patients with HCC
    Authors: H Sun, J Xu, Q Huang, M Huang, K Li, K Qu, H Wen, R Lin, M Zheng, H Wei, W Xiao, R Sun, Z Tian, C Sun
    Cancer Res., 2018-09-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Assessment of Wound-Healing Properties of Medicinal Plants: The Case of Phyllanthus muellerianus
    Authors: YD Boakye, C Agyare, GP Ayande, N Titiloye, EA Asiamah, KO Danquah
    Front Pharmacol, 2018-08-21;9(0):945.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  9. Lesional CD4+ IFN-gamma + cytotoxic T lymphocytes in IgG4-related dacryoadenitis and sialoadenitis
    Authors: Takashi Maehara, Hamid Mattoo, Miho Ohta, Vinay S Mahajan, Masafumi Moriyama, Masaki Yamauchi et al.
    Annals of the Rheumatic Diseases
  10. LAP degradation product reflects plasma kallikrein-dependent TGF-beta activation in patients with hepatic fibrosis
    Authors: Mitsuko Hara, Akiko Kirita, Wakako Kondo, Tomokazu Matsuura, Keisuke Nagatsuma, Naoshi Dohmae et al.
    SpringerPlus
  11. Induction of latency-associated peptide (transforming growth factor-beta(1)) expression on CD4+ T cells reduces Toll-like receptor 4 ligand-induced tumour necrosis factor-alpha production in a transforming growth factor-beta-dependent manner.
    Authors: Boswell S, Sharif S, Alisa A, Pereira SP, Williams R, Behboudi S
    Immunology, 2011-03-23;133(3):278-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Suppressive properties of human CD4+CD25+ regulatory T cells are dependent on CTLA-4 expression.
    Authors: Birebent B, Lorho R, Lechartier H, de Guibert S, Alizadeh M, Vu N, Beauplet A, Robillard N, Semana G
    Eur. J. Immunol., 2004-12-01;34(12):3485-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
    Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2004-09-16;105(2):750-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization

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