Human LC3B Antibody

Catalog #: MAB11606 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11606-100
MAB11606-SP

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Detection of Human LC3B by Western Blot.

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Human LC3B Antibody Summary

Species Reactivity

Human

Specificity

Detects a synthetic peptide specific for human LC3B around amino acid 10 in Direct ELISA.

Source

Monoclonal Mouse IgG_2A Clone # 1082854

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic Peptide
Accession # Q9GZQ8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

A172 human glioblastoma cell line untreated (-) or treated (+) with 50 μM Chloroquine for 18 hours

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human LC3B by Western Blot. Western Blot shows lysates of A172 human glioblastoma cell line untreated (-) or treated (+) with 50µM Chloroquine for 18 hours. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human LC3B Monoclonal Antibody (Catalog # MAB11606) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for LC3B at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LC3B

Human Microtubule-associated Protein (MAP) Light Chain 3 (LC3) A is a121 amino acid (aa) protein with a predicted molecular weight of 14 kDa. It is a member of the LC3 subfamily of Autophagy-related 8 (Atg8) proteins (1). The LC3 subfamily also includes LC3B andLC3C. LC3 exhibits 100% aa sequence identity with its mouse and rat orthologs, and is orthologous to the yeast autophagy-related protein Atg8. Atg8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. LC3 was originally described as part is part of a complex that includes heavy and light chains comprising the MAP1 family of microtubule regulatory proteins (3). However, LC3 has gained attention for MAP1-independent functions in autophagy. LC3 utilizes a ubiquitin-like conjugation system that includes E1-, E2-, and E3-like enzymes to covalently attach phosphatidylethanolamine (PE) to its C-terminus, incorporating it into the phagophore membrane during the early stages of autophagasome formation (4). Recruitment of LC3 to the phagophore may promote membrane elongation (4,5). It may also be involved in cargo recruitment to autophagosomes (1). LC3 is often used as a marker of autophagy.

References

Shpilka, T. et al. (2011) Genome Biol. 12:226.
He, H. et al. (2003) J. Biol. Chem. 278:29278.
Kuznetsov, S.A. & V.I. Gelfand (1987) FEBS Let. 212:145.
Weidberg, H. et al. (2011) Ann Rev. Biochem. 80:125.
Weidberg, H. et al. (2010) EMBO J. 29:1792.

Long Name

Microtubule-associated Protein 1 Light Chain 3 beta

Entrez Gene IDs

81631 (Human); 67443 (Mouse); 64862 (Rat)

Alternate Names

Apg8b; ATG8F; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; lc3b autophagy marker; LC3B; lc3-i, ii; LC3II; MAP1 light chain 3-like protein 2; MAP1A/1BLC3; MAP1A/MAP1B LC3 B; MAP1LC3B; MAP1LC3B-a; microtubule associated protein 1 light chain 3 beta; microtubule associated protein 3 b; microtubule associated protein 3 beta; microtubule-associated proteins 1A/1B light chain 3; microtubule-associated proteins 1A/1B light chain 3B