Human LILRA3/CD85e Alexa Fluor® 532-conjugated Antibody

Catalog #: AF2574X Datasheet / COA / SDS

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AF2574X-100UG

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All LILRA3/CD85e Antibodies

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LILRA3/CD85e Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human LILRA3/CD85e in direct ELISAs. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) ILT2, rhILT4, ILT5 is observed, and less than 5% cross-reactivity with rhLIR5 and rhLIR7 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LILRA3/CD85e
Arg18-Glu439
Accession # Q8N6C8

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Intracellular Staining by Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: LILRA3/CD85e

Human LIR-4 (Leukocyte Ig-like receptor #4; also ILT6 and LILRA3) is an Ig superfamily member that belongs to the leukocyte receptor complex/cluster. Mature LIR-4 is 416 amino acids in length and contains four C2-type Ig-like domains. Unlike other LIR family members, LIR-4 is actively secreted. At least one alternate splice form is known which shows a 64 amino acid deletion in the C-terminal half of the second Ig-like domain. Multiple alleles exist in human, and 5-10% of the population have a defective gene which produces no LIR-4 product. Rodent LIR-4 has not been reported.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM and ITIM Domains), Member 3

Entrez Gene IDs

11026 (Human)

Alternate Names

CD85 antigen-like family member E; CD85e antigen; CD85e; HM31; HM43; ILT6; ILT-6; ILT6CD85e; Immunoglobulin-like transcript 6; leucocyte immunoglobulin-like receptor; Leukocyte immunoglobulin-like receptor 4; leukocyte immunoglobulin-like receptor A3; leukocyte immunoglobulin-like receptor subfamily A member 3; leukocyte immunoglobulin-like receptor, subfamily A (without TM domain), member3; LILRA3; LIR4; LIR4CD85E; LIR-4HM43e3; Monocyte inhibitory receptor HM43/HM31