Human LILRA4/CD85g/ILT7 PE‑conjugated Antibody

R&D Systems | Catalog # FAB6287P

R&D Systems
Discontinued Product
FAB6287P has been discontinued. View all LILRA4/CD85g/ILT7 products.

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 656688
View all LILRA4/CD85g/ILT7 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ILT7/CD85g
Glu24-Asn446
Accession # P59901

Specificity

Detects human ILT7/CD85g in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) LILRA5, ILT2, 3, 4, 5, or 6 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human LILRA4/CD85g/ILT7 PE‑conjugated Antibody

Detection of LILRA4/CD85g/ILT7 antibody in Human Whole Blood Monocytes antibody by Flow Cytometry.

Detection of LILRA4/CD85g/ILT7 in Human Whole Blood Monocytes by Flow Cytometry.

Human whole blood monocytes were stained with Mouse Anti-Human LILRA4/CD85g/ILT7 PE-conjugated Monoclonal Antibody (Catalog # FAB6287P) and Goat Anti-Human DLEC/CLEC4C/BDCA-2 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1376A). Quadrant markers were set based on control antibody staining (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human LILRA4/CD85g/ILT7 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human whole blood monocytes

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: LILRA4/CD85g/ILT7

LILRA4 (Leukocyte Immunoglobulin-Like Receptor subfamily A member 4; also ILT-7 and CD85g) is a 35-38 kDa member of the Ig superfamily of molecules. Mature LILRA4 is a 476 amino acid (aa) type I transmembrane glycoprotein that possesses four C2-type Ig-like domains plus a nitrosylated tyrosine in its extracellular region (aa 24-446). There is one alternative start site at Met67 for human LILRA4, and rodent possesses no known gene counterpart for primate LILRA4. LILRA4 reveals a very restricted pattern of expression, being found only on/in microglia and immature plasmacytoid dendritic cells (pDC). On the cell surface, LILRA4 complexes with FceRI, and appears to generally transmit downmodulating signals. Although controversial, one potential ligand for LILRA4 has been identified and found to be BST2/CD317. Initially, it was suggested that LILRA4 served as a brake on type I interferon (IFN-a/b) production. In theory, viral nucleic acid detection by TLR-7 or -9 in pDC would first induce IFN production, followed by IFN-stimulated upregulation of BST2 on fibroblasts and endothelium, and ending with BST2 binding to LILRA4 on pDC, downregulating the earlier virus-based stimulation of IFN secretion. While this may occur, an additional theory has been proposed for LILRA4 that is also based on TLR-7 and -9 signaling. In this case, LILRA4 is "activated" following TLR-7/-9 ligation and immature circulating pDC will differentiate into APCs. But in the absence of LILRA4 activation after TLR-7/-9 stimulation, pDC will differentiate into fully functional type I interferon producing cells.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 4

Alternate Names

CD85g, ILT7

Entrez Gene IDs

23547 (Human)

Gene Symbol

LILRA4

UniProt

P59901 (Human)

Additional LILRA4/CD85g/ILT7 Products

Product Documents for Human LILRA4/CD85g/ILT7 PE‑conjugated Antibody

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Product Specific Notices for Human LILRA4/CD85g/ILT7 PE‑conjugated Antibody

For research use only

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