Human LILRB5/CD85c/LIR-8 Alexa Fluor® 750-conjugated Antibody

Catalog #: AF3065S Datasheet / COA / SDS

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AF3065S-100UG

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Human LILRB5/CD85c/LIR-8 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human LILRB5/CD85c/LIR-8 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) LILRA4/ILT7 is observed and less than 15% cross-reactivity with rhILT3 and rhILT5 is obser

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LILRB5/CD85c/LIR-8
Arg18-His456
Accession # O75023

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: LILRB5/CD85c/LIR-8

Human LIR-8 (also known as CD85c and LILRB5) is a 587 amino acid transmembrane protein of the family of Leukocyte Immunoglobin-like Receptors. LIR-8 has a long N-terminus extracellular domain with four C2-type lg-like domains, and a short cytoplasmic tail with two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) typically involved in modulation of cellular responses. Human LIR-8 is expressed in natural killer (NK) cells. There are at least two other known isoforms, one with 53 aa longer cytoplasmic domain, the other with a 100 aa deletion affecting one of the lg-like extracellular domains.

Long Name

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM And ITIM Domains), Member 5

Entrez Gene IDs

10990 (Human); 102129427 (Cynomolgus Monkey)

Alternate Names

CD85 antigen-like family member C; CD85c antigen; CD85c; Leukocyte immunoglobulin-like receptor 8; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB5; LIR8; LIR-8; LIR8CD85C; LIR-8leukocyte immunoglobulin-like receptor subfamily B member 5; member 5