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Human LPAR1/LPA1/EDG-2 Antibody

Catalog #: MAB99631
1 Citation Datasheet / COA / SDS

Discontinued Product

MAB99631 has been discontinued.
View all LPAR1/LPA1/EDG-2 products.
Detection of LPAR1/LPA1/EDG-2 in Human PBMC Monocytes by Flow Cytometry.
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Human LPAR1/LPA1/EDG-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LPAR1/LPA1/EDG-2 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 1020714
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human LPAR1/LPA1/EDG-2
Met1-Lys50
Accession # Q92633
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
Human PBMC Monocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of LPAR1/LPA1/EDG-2 antibody in Human PBMC Monocytes antibody by Flow Cytometry. View Larger

Detection of LPAR1/LPA1/EDG-2 in Human PBMC Monocytes by Flow Cytometry. Human PBMC monocytes were stained with Mouse Anti-Human LPAR1/LPA1/EDG-2 Monoclonal Antibody (Catalog # MAB99631, filled histogram) or Mouse IgG1 Isotype Control Antibody (MAB002, open histogram) followed by anti-Mouse IgG APC-conjugated secondary antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LPAR1/LPA1/EDG-2

Lysophosphatidic acid receptor 1, LPAR1, also known as EDG-2, is a G protein-coupled receptor that binds the lipid signaling molecule lysophosphatidic acid (LPA). EDG molecules are G protein-coupled receptors that bind plasma lysophospholipids. The EDG family consists of two subfamilies; the S1P (sphingosine-1-phosphate) subfamily consisting of EDG-1, 3, 5, 6, and 8, and the LPA subfamily that contains EDG-2, 4 and 7. The S1P family regulates essential cellular processes such as proliferation, migration, cytoskeletal organization, and differentiation.

Long Name
Lysophosphatidic Acid Receptor 1
Entrez Gene IDs
1902 (Human); 14745 (Mouse)
Alternate Names
EDG2; edg-2; EDG2Lysophosphatidic acid receptor Edg-2; endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor2,; GPCR26; GPR26; LPA receptor 1; LPA1; LPA-1; LPA1vzg-1; LPAR1; lysophosphatidic acid receptor 1; Mrec1.3; rec.1.3; ventricular zone gene 1; VZG1; vzg-1

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Citation for Human LPAR1/LPA1/EDG-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. LPA1-mediated inhibition of CXCR4 attenuates CXCL12-induced signaling and cell migration
    Authors: Jong Min Hong, Jin-Woo Lee, Dong-Seung Seen, Jae-Yeon Jeong, Won-Ki Huh
    Cell Commun Signal
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot

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