Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Immunocytochemistry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 125518
View all Mer Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Mer
Met1-Ala499
Accession # AAB60430

Specificity

Detects human Mer in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Axl, rhDtk, or recombinant mouse Mer is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Mer APC‑conjugated Antibody

Detection of Mer antibody in HepG2 Human Cell Line antibody by Flow Cytometry.

Detection of Mer in HepG2 Human Cell Line by Flow Cytometry.

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Mer APC-conjugated Monoclonal Antibody (Catalog # FAB8912A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of Mer antibody in U937 Human Cell Line antibody by Flow Cytometry.

Detection of Mer in U937 Human Cell Line by Flow Cytometry.

U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Mer APC-conjugated Monoclonal Antibody (Catalog # FAB8912A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Mer APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HepG2 human hepatocellular carcinoma cell line and U937 human histiocytic lymphoma cell line

Reviewed Applications

Read 1 review rated 5 using FAB8912A in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Mer

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Recent studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions.

References

  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Long Name

Receptor Tyrosine Protein Kinase Mer

Alternate Names

c-Eyk, C-mer, MerTK

Entrez Gene IDs

10461 (Human); 17289 (Mouse); 102143798 (Cynomolgus Monkey)

Gene Symbol

MERTK

UniProt

AAB60430

Additional Mer Products

Product Documents for Human Mer APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human Mer APC‑conjugated Antibody

For research use only

Citations for Human Mer APC‑conjugated Antibody

Customer Reviews for Human Mer APC‑conjugated Antibody (1)

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  • Human Mer APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs) and Peripheral blood monocytes
    Species: Human
    Verified Customer | Posted 09/20/2018
    Human Mer APC‑conjugated Antibody FAB8912A

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