ASAM (Adipocyte-specific adhesion molecule; also ACAM, CLMP, Asp5 and CXADR-like protein) is a 44-48 kDa member of the CTX family of molecules. It is expressed on adipocytes as well as select epithelial cells such as respiratory and intestinal epithelium, keratinocytes and Sertoli cells. ASAM appears to contribute to tight junction formation, and may promote the transition of preadipocytes to adipocytes. Mature mouse ASAM is a 356 amino acid (aa) type I transmembrane glycoprotein. It contains a 217 aa extracellular region (aa 18-234) that is characterized by the presence of two C2 type Ig-like domains (aa 18-126 and 134-223), and possesses a C-terminal 118 aa cytoplasmic domain. The extracellular region of mouse ASAM (aa 18-234) shares 97% and 100% aa sequence identity with human and rat ASAM, respectively.
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot, Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse ASAM
Thr18-Ala234
Accession # Q8R373
Thr18-Ala234
Accession # Q8R373
Specificity
Detects human and mouse recombinant ASAM in direct ELISAs. Detects human and mouse ASAM in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for ASAM Antibody
Detection of Mouse ASAM by Western Blot.
Western blot shows lysates of mouse adipose (uterus) tissue and mouse brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse ASAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7356) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ASAM at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of ASAM in RAW 264.7 Mouse Cell Line by Flow Cytometry.
RAW 264.7 mouse monocyte/macrophage cell line was stained with Sheep Anti-Mouse ASAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7356, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).
Detection of ASAM in T98G Human Cell Line by Flow Cytometry.
T98G human glioblastoma cell line was stained with Sheep Anti-Mouse ASAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7356, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).Applications for ASAM Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: T98G human glioblastoma cell line and RAW 264.7 mouse monocyte/macrophage cell line
Sample: T98G human glioblastoma cell line and RAW 264.7 mouse monocyte/macrophage cell line
Western Blot
1 µg/mL
Sample: Mouse adipose (uterus) tissue and mouse brain (cortex) tissue
Sample: Mouse adipose (uterus) tissue and mouse brain (cortex) tissue
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: ASAM
Long Name
Adipocyte-specific Adhesion Molecule
Alternate Names
ACAM, Asp5, CLMP
Gene Symbol
CLMP
UniProt
Additional ASAM Products
Product Documents for ASAM Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for ASAM Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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