Human/Mouse ATG4A Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB4324N Datasheet / COA / SDS

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FAB4324N-100UG

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Human/Mouse ATG4A Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human ATG4A in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human ATG4B is observed.

Source

Monoclonal Mouse IgG2b Clone # 514914

Purification

Protein A or G purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ATG4A isoform 1
Glu2-Val398
Accession # Q8WYN0

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ATG4A

Autophagy related protein 4 homolog A (ATG4A), also known as autophagin 2, is a 50 kDa cytosolic cysteine protease mostly found in skeletal muscle and brain. It belongs to the autophagin protein family which is involved in autophagy, a biological process in which endogenous proteins and damaged organelles are destroyed. Human ATG4A shares 100%, 94% and 93% identity with that of chimpanzee, dog and mouse.

Long Name

ATG4 Autophagy Related 4 Homolog A

Entrez Gene IDs

115201 (Human); 666468 (Mouse)

Alternate Names

APG4 autophagy 4 homolog A (S. cerevisiae); Apg4a; ATG4 autophagy related 4 homolog A (S. cerevisiae); ATG4A; Atg4al; Autl2; AUT-like 2 cysteine endopeptidase; AUT-like 2, cysteine endopeptidase (S. cerevisiae); Autophagin 2; autophagin-2; cysteine endopeptidase; cysteine protease ATG4A; EC 3.4.22; EC 3.4.22.-; hAPG4A