Human/Mouse CCR6 PE‑conjugated Antibody

Detection of CCR6 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Human/Mouse CCR6 PE-conjugated Monoclonal Antibody (Catalog # FAB590P) or (B) Rat IgG_2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Detection of CCR6 in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Rat Anti-Human/Mouse CCR6 PE-conjugated Monoclonal Antibody (Catalog # FAB590P) or (B) Rat IgG_2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Detection of CCR6 by Flow Cytometry

gamma delta T cells in LNs can be divided into distinct subsets using CD62L and CD44 expression. (a) CD62L and CD44 expression in alpha beta CD8+ and gamma delta T cells from skin-draining peripheral (top) and gut-draining mesenteric (bottom) LNs of untreated WT mice. Numbers show frequencies of respective gates (n = 6–9 mice in 5 independent experiments, mean ± SD). (b) Total cell numbers of CD62LhiCD44lo, CD62LhiCD44hi and CD62LloCD44hi alpha beta CD8+ and gamma delta T cells from skin-draining peripheral (top) and gut-draining mesenteric (bottom) LNs of untreated WT mice (n = 5 mice in 2 independent experiments, mean ± SD, one-way ANOVA with Tukey’s multiple comparisons test, **P < 0.01; ***P < 0.001; ns: not significant). (c) CD27 and CCR6 expression in CD62LhiCD44lo, CD62LhiCD44hi and CD62LloCD44hi gamma delta T cells from skin-draining peripheral (top) and gut-draining mesenteric (bottom) LNs of untreated WT mice. Numbers show frequencies of respective gates (n = 5 mice in 2 independent experiments, mean ± SD). (d) Expression levels of S1pr1, Klf2, Ccr7 in CD62LhiCD44lo, CD62LhiCD44hi and CD62LloCD44hi alpha beta CD8+ and gamma delta T cells from mLN of untreated WT mice (n = 4 pools of 10–12 mice in 4 independent experiments, mean ± SD, one-way ANOVA with Tukey’s multiple comparisons test, AU: arbitrary units, *P < 0.05; **P < 0.01; ***P < 0.001; ns: not significant). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29895956), licensed under a CC-BY license. Not internally tested by R&D Systems.