GATA-1 Antibody (234737)

R&D Systems | Catalog # MAB17791

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 234737
View all GATA-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GATA-1
Met1-Ser413
Accession # P15976

Specificity

Detects endogenous human and mouse GATA-1 in Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for GATA-1 Antibody (234737)

Detection of Human/Mouse GATA‑1 antibody by Western Blot.

Detection of Human/Mouse GATA‑1 by Western Blot.

Western blot shows lysates of TS1 mouse helper T cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse GATA-1 Monoclonal Antibody (Catalog # MAB17791) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for GATA-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Applications for GATA-1 Antibody (234737)

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: TS1 mouse helper T cell line and K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GATA-1

GATA-1 is the founding member of the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue‑specific programs of gene expression and morphogenesis. GATA-1 is expressed in blood forming cells. It interacts with several additional proteins to activate or repress gene expression and is essential for erythropoiesis.

Alternate Names

GATA1

Entrez Gene IDs

2623 (Human); 14460 (Mouse)

Gene Symbol

GATA1

UniProt

P15976

Additional GATA-1 Products

Product Documents for GATA-1 Antibody (234737)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for GATA-1 Antibody (234737)

For research use only

Citations for GATA-1 Antibody (234737)

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