Glucagon Antibody (181402) [NL557]

R&D Systems | Catalog # NL1249R

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry

Label

NL557 (Excitation = 557 nm, Emission = 574 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 181402
View all Glucagon Primary Antibodies »

Product Specifications

Immunogen

Human Glucagon synthetic peptide

Specificity

Detects human and mouse Glucagon.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Glucagon Antibody (181402) [NL557]

Glucagon antibody in beta TC-6 Mouse Cell Line by Immunocytochemistry (ICC).

Glucagon in beta TC-6 Mouse Cell Line.

Glucagon was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using 10 µg/mL Human/Mouse Glucagon NorthernLights™-557 Conjugated Monoclonal Antibody (Catalog # NL1249R) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Glucagon in U2OS cells (Positive) & RT‑4 cells (Negative).

Glucagon was detected in immersion fixed U2OS human osteosarcoma cells (Positive) & absent in RT‑4 human urinary bladder transitional cell papilloma (Negative) using Mouse Anti-Human/Mouse Glucagon NorthernLights™ NL557-conjugated Monoclonal Antibody (Catalog # NL1249R) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Nucleolus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Glucagon Antibody (181402) [NL557]

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion-fixed beta TC-6 mouse beta cell insulinoma cell line

Formulation, Preparation, and Storage

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Glucagon

Glucagon is a 29 amino acid residue pancreatic peptide hormone. It stimulates gluconeogenesis and glycogenolysis to counter the glucose-lowering action of insulin.

Alternate Names

GCG, GRPP

Entrez Gene IDs

2641 (Human); 14526 (Mouse); 24952 (Rat)

Gene Symbol

GCG

Additional Glucagon Products

Product Documents for Glucagon Antibody (181402) [NL557]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Glucagon Antibody (181402) [NL557]

For research use only

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