Glucagon Antibody (181402) [NL557]
R&D Systems | Catalog # NL1249R
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry
Label
NL557 (Excitation = 557 nm, Emission = 574 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 181402
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Product Specifications
Immunogen
Human Glucagon synthetic peptide
Specificity
Detects human and mouse Glucagon.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Glucagon Antibody (181402) [NL557]
Glucagon in beta TC-6 Mouse Cell Line.
Glucagon was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using 10 µg/mL Human/Mouse Glucagon NorthernLights™-557 Conjugated Monoclonal Antibody (Catalog # NL1249R) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Glucagon in U2OS cells (Positive) & RT‑4 cells (Negative).
Glucagon was detected in immersion fixed U2OS human osteosarcoma cells (Positive) & absent in RT‑4 human urinary bladder transitional cell papilloma (Negative) using Mouse Anti-Human/Mouse Glucagon NorthernLights™ NL557-conjugated Monoclonal Antibody (Catalog # NL1249R) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Nucleolus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Glucagon Antibody (181402) [NL557]
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion-fixed beta TC-6 mouse beta cell insulinoma cell line
Sample: Immersion-fixed beta TC-6 mouse beta cell insulinoma cell line
Formulation, Preparation, and Storage
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: Glucagon
Additional Glucagon Products
Product Documents for Glucagon Antibody (181402) [NL557]
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Glucagon Antibody (181402) [NL557]
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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