Golgi Glycoprotein 1/GLG1 Antibody (858238)

R&D Systems | Catalog # MAB78791

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 858238
View all Golgi Glycoprotein 1/GLG1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Golgi Glycoprotein 1/GLG1
Lys1048-Asn1145
Accession # Q92896

Specificity

Detects human Golgi Glycoprotein 1/GLG1 in direct ELISA. Detects human and mouse GLG1 in Western Blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Golgi Glycoprotein 1/GLG1 Antibody (858238)

Detection of Human and Mouse Golgi Glycoprotein 1/GLG1 antibody by Western Blot.

Detection of Human and Mouse Golgi Glycoprotein 1/GLG1 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse Golgi Glycoprotein 1/GLG1 Monoclonal Antibody (Catalog # MAB78791) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Golgi Glycoprotein 1/GLG1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Golgi Glycoprotein 1/GLG1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Golgi Glycoprotein 1/GLG1 in HeLa Human Cell Line.

Golgi Glycoprotein 1/GLG1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse Golgi Glycoprotein 1/GLG1 Monoclonal Antibody (Catalog # MAB78791) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Golgi Glycoprotein 1/GLG1 Antibody (858238)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Western Blot

2 µg/mL
Sample: MCF‑7 human breast cancer cell line and Hepa 1‑6 mouse hepatoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Golgi Glycoprotein 1/GLG1

GLG1 (Golgi complex-Localized Glycoprotein 1), also known as CFR1, E-Selectin Ligand-1/ESL-1, MG-160 and Cys-rich FGF Receptor, is a 150-160 kDa (reducing; 130 kDa nonreducing) glycoprotein. It is expressed in both Golgi and/or the cell membrane of multiple cell types, including neutrophils (from rodents; not humans), liver stellate cells, neurons, cardiac myocytes, monocytes and bronchial epithelial cells. In the blood, GLG1/ESL-1 collaborates with PSGL-1 to mediate leukocyte binding to endothelial cell surfaces. PSGL-1 initiates leukocyte tethering while GLG1 promotes slow rolling. GLG1 also serves as an intra-Golgi receptor for multiple FGFs, including FGF-1, -2, -4, -18 and possibly -3, and as a component of an unusual latent TGF-beta complex. Mature human GLG1 is an 1150 amino acid (aa) type I transmembrane protein. It contains a 1116 aa extracellular/luminal region (aa 30-1145) plus a short 13 aa cytoplasmic segment. The extracellular region possesses a 16 aa poly-Gln segment followed by 16 Cys-rich repeats (aa 116-1101). There are three potential isoform variants, one of which possess a 24 aa extension at the C‑terminus, a second that couples the aforementioned C‑terminal extension to a deletion of aa 147-157, and a third that contains a 14 aa substitution for aa 685-1179. It is suggested that the longer C‑terminus retains GLG1 in the Golgi, while shorter cytoplasmic segments allow for presentation at the cell membrane. Over aa 1048‑1145, human and mouse are identical in aa sequence.

Alternate Names

CFR-1, ESL-1, GLG1, MG160

Entrez Gene IDs

2734 (Human); 20340 (Mouse); 29476 (Rat)

Gene Symbol

GLG1

UniProt

Q92896

Additional Golgi Glycoprotein 1/GLG1 Products

Product Documents for Golgi Glycoprotein 1/GLG1 Antibody (858238)

Certificate of Analysis

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Product Specific Notices for Golgi Glycoprotein 1/GLG1 Antibody (858238)

For research use only

Citations for Golgi Glycoprotein 1/GLG1 Antibody (858238)

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