GSK-3 alpha/beta Antibody (833916)

R&D Systems | Catalog # MAB25063

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 833916
View all GSK-3 alpha/beta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GSK-3 beta
Asp200-Phe305
Accession # P49841

Specificity

Detects human GSK-3 beta in direct ELISAs and human GSK-3 alpha and GSK-3 beta in Western blots. Detects human and mouse GSK-3 alpha / beta in immunocytochemistry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for GSK-3 alpha/beta Antibody (833916)

Detection of Human GSK 3a and GSK-3 beta antibody by Western Blot.

Detection of Human GSK 3 alpha and GSK‑3 beta by Western Blot.

Western blot shows lysates of U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GSK-3a/ beta Monoclonal Antibody (Catalog # MAB25063) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for GSK-3a at approximately 46 and GSK-3 beta at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GSK-3a/ beta antibody in HT-29 Human Cell Line by Immunocytochemistry (ICC).

GSK‑3 alpha / beta in HT‑29 Human Cell Line.

GSK-3a/ beta was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human GSK-3a/ beta Monoclonal Antibody (Catalog # MAB25063) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
GSK-3a/ beta antibody in NIH-3T3 Mouse Cell Line by Immunocytochemistry (ICC).

GSK‑3 alpha / beta in NIH‑3T3 Mouse Cell Line.

GSK-3a/ beta was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Mouse Anti-Human GSK-3a/ beta Monoclonal Antibody (Catalog # MAB25063) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for GSK-3 alpha/beta Antibody (833916)

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed HT‑29 human colon adenocarcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line

Western Blot

2 µg/mL
Sample: U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GSK-3 alpha/beta

Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta ) share 85% amino acid identity. GSK-3 beta, inhibited by phosphorylation at S9 by Akt, is involved in energy metabolism, body pattern formation, and neuronal cell development.

Long Name

Glycogen Synthase Kinase 3

Alternate Names

DKFZp686D0638, EC 2.7.11, EC 2.7.11.26, glycogen synthase kinase 3 alpha, glycogen synthase kinase-3 alpha, GSK-3 alpha

UniProt

P49841

Additional GSK-3 alpha/beta Products

Product Documents for GSK-3 alpha/beta Antibody (833916)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for GSK-3 alpha/beta Antibody (833916)

For research use only

Citations for GSK-3 alpha/beta Antibody (833916)

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Protocols

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