Human/Mouse Lysine (K)‑specific Demethylase 4A/KDM4A Alexa Fluor™ Plus 680‑conjugated Antibody

R&D Systems | Catalog # AF6434AFP680

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Polyclonal Sheep IgG
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Product Specifications

Specificity

Detects human KDM4A in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human KDM4C is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Lysine (K)-specific Demethylase 4A/KDM4A

KDM4A (Lysine [K]-specific demethylase 4A; also known as JMJD2A) is a 155-160 kDa member of the JHDM3 histone demethylase family of enzymes. It is ubiquitously expressed and interacts with the histone deacetylase proteins HDAC-1, -2, and -3. KDM4A specifically demethylates trimethylated lysines on histone H3 at Lys9 and Lys36. Transcriptional repression occurs when it interacts with NCOR1. Human KDM4A is 1064 amino acids (aa) in length (SwissProt #:O75164). It contains one jumonji N domain (aa 13-55), a catalytic jumonji C domain (aa 175-291), two PHD zinc finger regions (aa 709-855), and two TUDOR methylated-histone binding domains (aa 897-1011). Over aa 386-514, human KDM4A shares 93% amino acid sequence identity with mouse KDM4A.

Alternate Names

JHDM3A, JMJD2, JMJD2A, KDM4A, Lysine (K)specific Demethylase 4A

Entrez Gene IDs

9682 (Human); 230674 (Mouse); 313539 (Rat)

Gene Symbol

KDM4A

UniProt

Additional Lysine (K)-specific Demethylase 4A/KDM4A Products

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

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Protocols

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