Lysine (K)-specific Demethylase 4A/KDM4A Antibody

R&D Systems | Catalog # AF6434

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Rabbit

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Lysine (K)-specific Demethylase 4A/KDM4A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human KDM4A
Lys386-Ser514
Accession # AAH02558

Specificity

Detects human KDM4A in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human KDM4C is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Lysine (K)-specific Demethylase 4A/KDM4A Antibody

Detection of Human and Mouse Lysine (K)-specific Demethylase 4A/KDM4A antibody by Western Blot.

Detection of Human and Mouse Lysine (K)‑specific Demethylase 4A/KDM4A by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, THP-1 human acute monocytic leukemia cell line, and mouse embryo tissue. PVDF Membrane was probed with 1 µg/mL of Human Lysine (K)-specific Demethylase 4A/KDM4A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6434) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Lysine (K)-specific Demethylase 4A/KDM4A at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
KDM4A antibody in BG01V Human Cell Line by Immunocytochemistry (ICC).

KDM4A in BG01V Human Cell Line.

KDM4A was detected in immersion fixed endoderm-differentiated BG01V human embryonic stem cells using Human/Mouse KDM4A Affinity-purified Polyclonal Antibody (Catalog # AF6434) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of KDM4A by Immunocytochemistry/ Immunofluorescence

Detection of KDM4A by Immunocytochemistry/ Immunofluorescence

Expression patterns of H3K9 modifying enzymes in single-cell RNA-seq data. (A-B) Expression patterns of enzymes responsible for H3K9 methylation (A) and acetylation (B) in cells of the morula (day 3.0), ICM (day 3.5-4.0), and epiblast (day 5.0-6.0-6.6) cells. The dot size represents the percentage of cells expressing the gene of interest, while the color intensity reflects the average expression level across all cells. (C) Immunofluorescence imaging of EHMT1/2, SETDB1, KDM4AA, and HDAC2 in embryos at embryonic days E3.0, E3.5, E4.0, E5.0, E6.0, and E6.6, with DAPI counterstaining and SOX2 co-labeling. N = 3 experiments for each enzyme tested. Scale bars: 100 μm. (D) Violin plots representing the quantification of the fluorescent signal for each histone-modifying enzyme in SOX2+ cells. Each embryo is represented by a unique color, with n = 4 to 8 embryos per stage. All cells were quantified separately, with the mean of each embryo indicated with a larger dot. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39800758), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Lysine (K)-specific Demethylase 4A/KDM4A Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed endoderm-differentiated BG01V human embryonic stem cells

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, THP‑1 human acute monocytic leukemia cell line, and mouse embryo tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lysine (K)-specific Demethylase 4A/KDM4A

KDM4A (Lysine [K]-specific demethylase 4A; also known as JMJD2A) is a 155-160 kDa member of the JHDM3 histone demethylase family of enzymes. It is ubiquitously expressed and interacts with the histone deacetylase proteins HDAC-1, -2, and -3. KDM4A specifically demethylates trimethylated lysines on histone H3 at Lys9 and Lys36. Transcriptional repression occurs when it interacts with NCOR1. Human KDM4A is 1064 amino acids (aa) in length (SwissProt #:O75164). It contains one jumonji N domain (aa 13-55), a catalytic jumonji C domain (aa 175-291), two PHD zinc finger regions (aa 709-855), and two TUDOR methylated-histone binding domains (aa 897-1011). Over aa 386-514, human KDM4A shares 93% amino acid sequence identity with mouse KDM4A.

Alternate Names

JHDM3A, JMJD2, JMJD2A, KDM4A, Lysine (K)specific Demethylase 4A

Entrez Gene IDs

9682 (Human); 230674 (Mouse); 313539 (Rat)

Gene Symbol

KDM4A

UniProt

AAH02558

Additional Lysine (K)-specific Demethylase 4A/KDM4A Products

Product Documents for Lysine (K)-specific Demethylase 4A/KDM4A Antibody

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Product Specific Notices for Lysine (K)-specific Demethylase 4A/KDM4A Antibody

For research use only

Related Research Areas

Epigenetics

Citations for Lysine (K)-specific Demethylase 4A/KDM4A Antibody

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