NeuroD1 Antibody

R&D Systems | Catalog # AF2746

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat, Transgenic Mouse, Xenograft

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NeuroD1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NeuroD1
Ser154-Asp356
Accession # Q13562

Specificity

Detects NeuroD1 in direct ELISAs and Western blots. In these formats, approximately 5% cross-reactivity with recombinant human NeuroD2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NeuroD1 Antibody

NeuroD1 antibody in beta TC-6 Mouse Cell Line by Immunocytochemistry (ICC).

NeuroD1 in beta TC-6 Mouse Cell Line.

Neurogenic Differentiation factor 1 (NeuroD1) was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse NeuroD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2746) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of NeuroD1 by Immunohistochemistry

Detection of NeuroD1 by Immunohistochemistry

Organoid characterization(A) Representative bright-field image of an organoid on the seventh day of culture.(B and C) Immunostaining of a representative organoid for iOSNs (Gap43), HBCs (KRT5), and GBCs (SOX2+, KRT5-). (C) shows high-magnification images of (B).(D–F) Representative immunostaining of organoids for other neurogenic markers.(G) UMAP representation of single-cell RNA-seq of the organoid culture.(H) Stacked violin plots of key marker genes allowing cluster annotation in log scale.(I) Focused UMAP expression plots in the dotted area shown in (G), plotted in log-scale, representing the neurogenic cascade found in the organoids.(J) Histogram plot of the number of distinct ORs found in single cells identified as OSNs based on UCHL1, GAP43, and TUBB3. Image collected and cropped by CiteAb from the following open publication (https://linkinghub.elsevier.com/retrieve/pii/S2667237525000918), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of NeuroD1 by Immunohistochemistry

Detection of NeuroD1 by Immunohistochemistry

MCT8-deficient COs present an altered transcriptome.(A) Schematic of the protocol for COs’ maturation using platelet-derived growth factor AA and insulin-like growth factor 1. (B–E) Whole-mount immunolabeling of D65 WT COs containing differentiated NEUROD1+ neurons (B), GFAP+ neural precursor cells and GFAP-AQP4+ astrocytes (C and D), and OLIG2+ oligodendrocyte precursor cells and O4+ premyelinating oligodendrocytes (E); in blue is the nuclear staining with DAPI. B: scale bar 75 μm; C: scale bar 75 μm; inset in C: scale bar 25 μm; D: scale bar 40 μm; E: scale bar 75 μm; inset in E: scale bar 40 μm. Arrows in D indicate AQP4 staining in the membrane of GFAP+ cells. The arrow in the inset in E points to the cellular body of a representative oligodendrocyte; arrowheads point to its cellular processes. (F and G) Volcano plots showing the distribution of DEGs in WT vs. Mut1 (F) and in WT vs. Mut2 (G); each point represents the average of 3 (WT) or 2 (MCT8-deficient) samples consisting of 4 pooled COs for each transcript. (H) Venn comparison of WT vs. Mut1, WT vs. Mut2, and Mut1 vs. Mut2 DEGs. A common cluster (cluster E) between WT vs. Mut1 and WT vs. Mut2 containing 949 DEGs was identified. (I) Venn comparison of the 949 DEGs in cluster E shows similar transcriptomic changes in both MCT8-deficient COs. (J) Heatmap depicting the top 63 DEGs from cluster E. (K) KEGG pathway analysis of the top 63 DEGs in J. (L–O) Heatmaps of T3-regulated genes involved in cerebral cortex development (L), neural cell migration (M), astrocytes and myelination (N), and neurotransmitter receptors, transcription factors, potassium channels, and extracellular matrix protein (O). DEG thresholds (FDR ≤ 0.25 and fold-change ± 4) were identified by gene set–specific analysis in the Partek Flow platform. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38376950), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for NeuroD1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed beta TC-6 mouse beta cell insulinoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human NeuroD1

Reviewed Applications

Read 1 review rated 5 using AF2746 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NeuroD1

NeuroD1, also known as beta-cell E-box transactivator 2 (Beta 2), is a basic helix-loop-helix transcription factor. It is expressed during development in various neurons of the central and peripheral nervous systems. NeuroD1 is also expressed in beta-cells in the pancreas where it functions both as a transcriptional activator and repressor.

Long Name

Neurogenic Differentiation Factor 1

Alternate Names

BETA2, BHF-1, NIDDM

Entrez Gene IDs

4760 (Human)

Gene Symbol

NEUROD1

UniProt

Q13562

Additional NeuroD1 Products

Product Documents for NeuroD1 Antibody

Certificate of Analysis

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Product Specific Notices for NeuroD1 Antibody

For research use only

Citations for NeuroD1 Antibody

Customer Reviews for NeuroD1 Antibody (1)

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  • Human/Mouse NeuroD1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: olfactory epithelium
    Species: Mouse
    Verified Customer | Posted 06/08/2016
    NeuroD1 Antibody AF2746

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