Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 613441
View all NRAGE Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NRAGE
Arg73-Asn254
Accession # Q9Y5V3

Specificity

Detects human NRAGE in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human MAGE-A4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse NRAGE Antibody

Detection of Human, Mouse, and Rat NRAGE antibody by Western Blot.

Detection of Human, Mouse, and Rat NRAGE by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NRAGE Monoclonal Antibody (Catalog # MAB38352) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NRAGE at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
NRAGE antibody in C2C12 Mouse Cell Line by Immunocytochemistry (ICC).

NRAGE in C2C12 Mouse Cell Line.

NRAGE was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human NRAGE Monoclonal Antibody (Catalog # MAB38352) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse NRAGE Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed C2C12 mouse myoblast cell line

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NRAGE

NRAGE, also known as MAGE-D1 or Dlxin-1, is a ubiquitously expressed cytosolic protein that is a member of the melanoma-associated antigen (MAGE) family. The 778 aa human NRAGE protein contains a segment with 22 repeats of a W(P/Q)xPxx motif, followed by a MAGE domain. Human and mouse NRAGE share 87% aa identity.  In the brain, NRAGE interacts with p75NTR, blocks cell cycle progression, and facilitates NGF-dependent apoptosis.

Long Name

Neurotrophin Receptor-interacting MAGE Homolog

Alternate Names

Dlxin-1, MAGED1

Entrez Gene IDs

9500 (Human); 94275 (Mouse); 84469 (Rat)

Gene Symbol

MAGED1

UniProt

Q9Y5V3 (Human)

Additional NRAGE Products

Product Documents for Human/Mouse NRAGE Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse NRAGE Antibody

For research use only

Citations for Human/Mouse NRAGE Antibody

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Protocols

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