The Phospholipase C family consists of 13 isozymes within six subfamilies, PLC-delta, -beta, -gamma, -epsilon, -zeta, and -eta. PLC-gamma 2 (Phospholipase C gamma-2) contains 2 SH2 and 1 SH3 domains and is primarily limited to cells of hematopoietic lineage. PLC- gamma 2 is activated by receptor tyrosine kinases in response to growth factors, neurotransmitters, and hormones. Activated PLC- gamma 2 catalyzes the hydrolysis of phosphatidylinositol 4, 5-biphosphate to produce the second messengers inositol 1, 4, 5-triphosphate (IP3) and diacylglycerol (DAG). IP3 mobilizes the release of calcium while DAG activates protein kinase C. PLC- gamma 2 is involved in collagen induced signaling in platelets and antigen-dependent signaling in B-lymphocytes.
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Western Blot, Immunocytochemistry
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 346404
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Product Specifications
Immunogen
E. coli-derived recombinant human PLC-gamma 2
Pro400-Tyr600
Accession # P16885
Pro400-Tyr600
Accession # P16885
Specificity
Detects human and mouse PLC-gamma 2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for PLC-gamma 2 Antibody (346404)
Detection of Human/Mouse PLC-gamma 2 by Western Blot.
Western blot shows lysates of A20 mouse B cell lymphoma cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse PLC- gamma2 Monoclonal Antibody (Catalog # MAB3716) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PLC- gamma2 at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PLC-gamma 2 in Human PBMCs.
PLC- gamma2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human/Mouse PLC- gamma2 Monoclonal Antibody (Catalog # MAB3716) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for PLC-gamma 2 Antibody (346404)
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells
Sample: Immersion fixed human peripheral blood mononuclear cells
Western Blot
1 µg/mL
Sample: A20 mouse B cell lymphoma cell line and U937 human histiocytic lymphoma cell line
Sample: A20 mouse B cell lymphoma cell line and U937 human histiocytic lymphoma cell line
Reviewed Applications
Read 1 review rated 5 using MAB3716 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: PLC-gamma 2
Long Name
Phospholipase C gamma 2
Alternate Names
PLCG2, PLCgamma 2
Gene Symbol
PLCG2
UniProt
Additional PLC-gamma 2 Products
Product Documents for PLC-gamma 2 Antibody (346404)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for PLC-gamma 2 Antibody (346404)
For research use only
Related Research Areas
Citations for PLC-gamma 2 Antibody (346404)
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Customer Reviews for PLC-gamma 2 Antibody (346404) (1)
5 out of 5
1 Customer Rating
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Application: Western BlotSample Tested: Blood mononuclear cells (PBMCs)Species: HumanVerified Customer | Posted 04/22/2016
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Pathogen or Damage-activated C-Type Lectin Receptor Signaling Pathways
VEGF - VEGF R2 Signaling Pathways
