Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all RASSF2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human RASSF2
Lys100-Gly230
Accession # P50749

Specificity

Detects endogenous human and mouse RASSF2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse RASSF2 Antibody

Detection of Human RASSF2 antibody by Western Blot.

Detection of Human RASSF2 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, CEM human T-lymphoblastoid cell line, and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse RASSF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5639) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for RASSF2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
RASSF2 antibody in U937 Human Cell Line by Immunocytochemistry (ICC).

RASSF2 in U937 Human Cell Line.

RASSF2 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Goat Anti-Human/Mouse RASSF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5639) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human/Mouse RASSF2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed U937 human histiolytic lymphoma cell line

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, CEM human T-lymphoblastoid cell line, and Daudi human Burkitt's lymphoma cell line

Reviewed Applications

Read 1 review rated 5 using AF5639 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RASSF2

RASSF2 (Ras association domain-containing protein 2; also RASSF2A) is a 34‑36 kDa member of the RASSF family of proteins. It is widely expressed, and serves as a tumor suppressor by interacting with K-Ras in a GTP-dependent manner, and activating caspase-3 and inducing apoptosis. Human RASSF2 is 326 amino acids (aa) in length. It contains a bipartite NLS (aa 151‑167), a Ras-association domain (aa 176‑264) and a SARAH domain (aa 272‑319) that mediates homo- and heterotypic interactions. Multiple isoforms exist. One shows an insertion of 13 aa after Lys213, a second shows a three aa substitution for aa 305‑326, and a third shows a 20 aa substitution for aa 1‑96, accompanied by a three aa substitution for aa 231‑326. RASSF2 is often found down regulated in lung-derived tumor cell lines.

Long Name

Ras Association [RalGDS/AF-6] Domain Family Member 2

Alternate Names

RASFADIN

Entrez Gene IDs

9770 (Human); 215653 (Mouse); 311437 (Rat)

Gene Symbol

RASSF2

UniProt

P50749

Additional RASSF2 Products

Product Documents for Human/Mouse RASSF2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse RASSF2 Antibody

For research use only

Related Research Areas

Cell Cycle

Citations for Human/Mouse RASSF2 Antibody

Customer Reviews for Human/Mouse RASSF2 Antibody (1)

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  • Human/Mouse RASSF2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Melanoma tissue
    Species: Human
    Verified Customer | Posted 04/06/2022
    Tissue was cryopreserved
    Human/Mouse RASSF2 Antibody AF5639

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