Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, FACS

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgM Clone # O4
View all Oligodendrocyte Marker O4 Primary Antibodies »

Product Specifications

Immunogen

Bovine brain corpus callosum white matter

Specificity

Detects human, mouse, rat, and chicken Oligodendrocyte Marker O4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Oligodendrocyte Marker O4 Antibody (O4) [PE]

Detection of Oligodendrocyte Marker O4 antibody in Rat Cortical Stem Cells antibody by Flow Cytometry.

Detection of Oligodendrocyte Marker O4 in Rat Cortical Stem Cells by Flow Cytometry.

Rat cortical stem cells either differentiated (filled histogram) or undifferentiated (open histogram) were stained with Mouse Anti-Human/Mouse/Rat/ Chicken Oligodendrocyte Marker O4 PE‑conjugated Monoclonal Antibody (Catalog # FAB1326P). View our protocol for Staining Membrane-associated Proteins.

Applications for Oligodendrocyte Marker O4 Antibody (O4) [PE]

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Differentiated rat cortical stem cells

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Oligodendrocyte Marker O4

Oligodendrocytes are myelinating cells in the central nervous system (CNS) and form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O4 is an antigen on the surface of oligodendrocyte progenitors (1, 2). It has been commonly used as the earliest recognized marker specific for the oligodendroglial lineage (3-8).

References

  1. Schachner, M. et al. (1981) Dev. Biol. 83:328.
  2. Bansal, R. et al. (1989) J. Neurosci. Res. 24:548.
  3. Bansal, R. and Pfeiffer, S.E. (1989) Proc. Natl. Acad. Sci. USA 86:6181.
  4. Gard, A. et al. (1995) Dev. Biol. 167:596.
  5. Reynolds, R. and Hardy, R. (1997) J. Neurosci. Res. 47:455.
  6. Ono, K. et al. (1997) J. Neurosci. Res. 48:212.
  7. Pang, Y. et al. (2000) J. Neurosci. Res. 62:510.
  8. Cai, Z. et al. (2001) Brain Res. 898:126.

Additional Oligodendrocyte Marker O4 Products

Product Documents for Oligodendrocyte Marker O4 Antibody (O4) [PE]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Oligodendrocyte Marker O4 Antibody (O4) [PE]

For research use only

Related Research Areas

Glial Lineage Markers

Citations for Oligodendrocyte Marker O4 Antibody (O4) [PE]

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