Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Applications

Validated:

Knockout Validated, Immunohistochemistry, Western Blot

Cited:

Immunoprecipitation, ELISA Development (Capture)

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all FAK Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FAK
Asp213-Thr412
Accession # Q05397

Specificity

Detects human, mouse and rat FAK.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat FAK Antibody

Detection of Human, Mouse, and Rat FAK antibody by Western Blot.

Detection of Human, Mouse, and Rat FAK by Western Blot.

Western blot shows lysates of MCF‑7 human breast cancer cell line, HUVEC human umbilical vein endothelial cells, Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat FAK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4467) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for FAK at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

FAK antibody in Human Brain by Immunohistochemistry (IHC-P).

FAK in Human Brain.

FAK was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using 3 µg/mL Human/Mouse/Rat FAK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4467) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Shows Human FAK Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human FAK Specificity by Using Knockout Cell Line.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and FAK knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat FAK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4467) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FAK at approximately 135 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human FAK by Western Blot

Detection of Human FAK by Western Blot

ADAM9 affects auto‐/paracrine MEK/ERK signaling. (A) Western blot of phosphorylation of MEK1/2 (S217/221) and ERK1/2 (T202/204) in AsPC‐1 and MiaPaCa‐2 cells following ADAM9 expression silencing. In AsPC‐1 cells, we observed decreased pFAK (Y397), which was absent in MiaPaCa‐2 cells. Quantitation of pFAK, pMEK1/2, and pERK1/2 in AsPc‐1 and MiaPaCa‐2 cells from three independent experiments. (B) Western blot analysis shows decreased phosphorylation of MEK1/2 (S217/221) and ERK1/2 (T202/204) in HUVEC cells following 30 min of incubation with cancer cell conditioned medium from both AsPC‐1 and MiaPaCa‐2 cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30556643), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat FAK Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)

Knockout Validated

FAK is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in FAK knockout HEK293T cell line.

Western Blot

1 µg/mL
Sample: HUVEC human umbilical vein endothelial cells, MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FAK

Focal adhesion kinase 1 (FAK) is a ubiquitously expressed non-receptor protein tyrosine kinase that is concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This cellular localization is directed by a "Focal Adhesion Targeting" (FAT) sequence, a 125 amino acid sequence at the C-terminus. FAK plays an important role in migration, cell spreading, differentiation, cytoskeleton protein phosphorylation, apoptosis and acceleration of the G1 to S phase transition of the cell cycle. It associates with several different signaling proteins such as Src-family PTKs, p130Cas, Shc, Grb2, PI 3-kinase, and paxillin. This enables FAK to function within a network of integrin-stimulated signaling pathways leading to the activation of targets such as the ERK and JNK/mitogen-activated protein kinase pathways. FAK is also linked to oncogenes at biochemical and functional levels. Increased expression and/or activity of FAK in various tumors has been correlated with enhanced migration and invasiveness of human tumor cells in addition to promoting increased cell proliferation.

Long Name

Focal adhesion kinase 1

Alternate Names

FADK1, PTK2

Entrez Gene IDs

5747 (Human); 14083 (Mouse); 25614 (Rat)

Gene Symbol

PTK2

UniProt

Q05397 (Human)

Additional FAK Products

Product Documents for Human/Mouse/Rat FAK Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat FAK Antibody

For research use only

Citations for Human/Mouse/Rat FAK Antibody

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Protocols

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