Human/Mouse/Rat MKK3 Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB2515G Datasheet / COA / SDS

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FAB2515G-100UG

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Human/Mouse/Rat MKK3 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human MKK3 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human MKK6 is observed.

Source

Monoclonal Mouse IgG2b Clone # 275909

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human MKK3
Met1-Ser347
Accession # P46734

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: MKK3

Mitogen-activated protein kinase kinase 3 (MKK3), also known as MEK3, and the closely related MKK6 are activated by proinflammatory cytokines and environmental stresses. Activation occurs through phosphorylation by several upstream MAPK kinase kinases, including ASK1 and TAK1. Both MKK3 and MKK6 are dual specificity protein kinases, phosphorylating and activating the p38 MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Gly-Tyr.

Long Name

Mitogen-activated Protein Kinase Kinase 3

Entrez Gene IDs

5606 (Human); 26397 (Mouse); 303200 (Rat)

Alternate Names

MAP kinase kinase 3; MAP2K3; MAPK/ERK kinase 3; MAPKK 3; MAPKK3PRKMK3dual specificity mitogen-activated protein kinase kinase 3; MEK3; MEK3MEK 3; mitogen activated protein kinase kinase 3; mitogen-activated protein kinase kinase 3; MKK3; MKK3EC 2.7.12.2; PRKMK3