Human/Mouse/Rat Src Antibody
Human/Mouse/Rat Src Antibody Summary
Accession # P12931
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Rat Src by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Y3-Ag rat myeloid cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Src Monoclonal Antibody (Catalog # MAB3389) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Src at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Detection of Human Src by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, U937 human histiocytic lymphoma cell line (negative control), and HL‑60 human acute promyelocytic leukemia cell line (negative control). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Src Monoclonal Antibody (Catalog # MAB3389) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Src at approximately 60 kDa (as indicated). GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The Src family of proteins are intracellular tyrosine kinases involved in cell proliferation, differentiation, motility, and survival. Src family activity is regulated by tyrosine phosphorylation at two sites with opposing effects. Autophosphorylation in the activation loop of the kinase domain (Y419 of human Src) upregulates the enzyme, while phosphorylation in the C-terminal tail (Y530 of human Src) downregulates activity.
Citations for Human/Mouse/Rat Src Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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PhosY-secretome profiling combined with kinase-substrate interaction screening defines active c-Src-driven extracellular signaling
Authors: Backe, SJ;Votra, SD;Stokes, MP;Sebesty�n, E;Castelli, M;Torielli, L;Colombo, G;Woodford, MR;Mollapour, M;Bourboulia, D;
Sample Types: Whole Cells
Kaposi's sarcoma-associated herpesvirus induces rapid release of angiopoietin-2 from endothelial cells.
Authors: Ye F, Zhou F, Nithianantham S, Chandran B, Yu X, Weinberg A, Gao S
J Virol, 2013-03-27;87(11):6326-35.
Sample Types: Cell Lysates
Applications: Western Blot
Activin A functions as a Th2 cytokine in the promotion of the alternative activation of macrophages.
Authors: Ogawa K, Funaba M, Chen Y, Tsujimoto M
J. Immunol., 2006-11-15;177(10):6787-94.
Sample Types: Cell Culture Supernates
Applications: Western Blot
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