Thioredoxin Reductase 1/TRXR1 Antibody (489804)

R&D Systems | Catalog # MAB7428

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Western Blot, Simple Western, Simple Plex Automated Immunoassay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 489804
View all Thioredoxin Reductase 1/TRXR1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Thioredoxin Reductase 1/TRXR1
Met1-Ile497
Accession # Q16881

Specificity

Detects mouse Thioredoxin Reductase 1/TRXR1 in direct ELISAs and human, mouse, and rat Thioredoxin Reductase 1/TRXR1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Thioredoxin Reductase 1/TRXR1 Antibody (489804)

Detection of Human, Mouse, and Rat Thioredoxin Reductase 1/TRXR1 antibody by Western Blot.

Detection of Human, Mouse, and Rat Thioredoxin Reductase 1/TRXR1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Thioredoxin Reductase 1/TRXR1 Monoclonal Antibody (Catalog # MAB7428) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Thioredoxin Reductase 1/TRXR1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Thioredoxin Reductase 1/TRXR1 antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of Thioredoxin Reductase 1/TRXR1 in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse/Rat Thioredoxin Reductase 1/TRXR1 Monoclonal Antibody (Catalog # MAB7428, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Detection of ejep3nzpqdwhbintr7bf.jpg by Western Blot

Detection of ejep3nzpqdwhbintr7bf.jpg by Western Blot

TrxR1 knockdown decreases BCR/NF-kappa B pathway-related mRNA and protein expression. SUDHL2 and SUDHL4 cells were transfected with TrxR1 specific siRNA (siTrxR) and a scrambled siRNA (control). (A,B), RT-qPCR was performed 48 h after transfection to determine the mRNA expression of TrxR1, BTK, p65, and surviving in SUDHL2 and SUDHL4 cells. (C,D), Western blotting was performed 48 h after transfection to determine the protein levels of BTK and TrxR in SUDHL2 and SUDHL4 cells. (E,F), The BTK expression was detected after treating cells with the indicated [Au(d2pype)2]Cl after 24 h in SUDHL2 and SUDHL4 cells. B-actin was used as the loading control for BTK protein blots, and Vinculin was used as the loading control for the TrxR protein blots. Western blots are representative of 3 independent experiments. Significance levels are marked as * p < 0.05 and ** p < 0.005. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36830087), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of fz2ftlqdhdi4eujrfghl.jpg by Western Blot

Detection of fz2ftlqdhdi4eujrfghl.jpg by Western Blot

TrxR1 knockdown decreases BCR/NF-kappa B pathway-related mRNA and protein expression. SUDHL2 and SUDHL4 cells were transfected with TrxR1 specific siRNA (siTrxR) and a scrambled siRNA (control). (A,B), RT-qPCR was performed 48 h after transfection to determine the mRNA expression of TrxR1, BTK, p65, and surviving in SUDHL2 and SUDHL4 cells. (C,D), Western blotting was performed 48 h after transfection to determine the protein levels of BTK and TrxR in SUDHL2 and SUDHL4 cells. (E,F), The BTK expression was detected after treating cells with the indicated [Au(d2pype)2]Cl after 24 h in SUDHL2 and SUDHL4 cells. B-actin was used as the loading control for BTK protein blots, and Vinculin was used as the loading control for the TrxR protein blots. Western blots are representative of 3 independent experiments. Significance levels are marked as * p < 0.05 and ** p < 0.005. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36830087), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Thioredoxin Reductase 1/TRXR1 Antibody (489804)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

0.1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line

Reviewed Applications

Read 1 review rated 5 using MAB7428 in the following applications:

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Thioredoxin Reductase 1/TRXR1

Thioredoxin reductase 1 (TRXR1) is an approximately 70 kDa member of the class-I pyridine nucleotide-disulfide oxidoreductase family.  Human TRXR1 is 649 amino acids (aa) in length. Residues 151-152 constitute a propeptide that is deleted from the mature protein. Splicing variants produce five additional isoforms for human TRXR1. Isoform 2 has a 32 aa substitution for aa 107-138 and a deletion of aa 1-106. Isoform 3 has a deletion of aa 1-51 and a 49 aa substitution for aa 52-100. Isoform 4 is missing aa 1-98 and has a 3 aa substitution for aa 99-101. Isoform 5 has a deletion of aa 1-150. Residues 56-156 make up a glutaredoxin domain, and residues 520-632 constitute a pyridine nucleotide-disulphide oxidoreductase dimerization domain. In addition, there are three phosphotyrosines at positions 161, 163, and 281, and a selenocysteine at position 648. Human TRXR1 shares 74% and 70% aa sequence identity with mouse and rat TRXR1, respectively. Isoform 1 is involved in glutaredoxin activity as well as thioredoxin reductase activity, and it induces actin and tubulin polymerization, which leads to formation of cell membrane protrusions.  Isoform 4 has been shown to enhance the transcriptional activity of the beta receptor only. Finally, isoform 5 mediates cell death induced by a combination of interferon-beta and retinoic acid. Isoform 1 is expressed mostly in the Leydig cells, but also in the ovary, spleen, heart, liver, kidney, and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in the kidney, uterus, testis, ovary, prostate, placenta, and fetal liver.

Alternate Names

GRIM-12, KDRF, TR1, TRXR1, TRXRD1, TXNR, TXNRD1

Entrez Gene IDs

7296 (Human); 50493 (Mouse); 58819 (Rat)

Gene Symbol

TXNRD1

UniProt

Q16881

Additional Thioredoxin Reductase 1/TRXR1 Products

Product Documents for Thioredoxin Reductase 1/TRXR1 Antibody (489804)

Certificate of Analysis

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Product Specific Notices for Thioredoxin Reductase 1/TRXR1 Antibody (489804)

For research use only

Related Research Areas

Redox Enzymes

Citations for Thioredoxin Reductase 1/TRXR1 Antibody (489804)

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  • Human/Mouse/Rat Thioredoxin Reductase 1/TRXR1 Antibody
    Name: Dharmaraja Allimuthu
    Application: Western Blot
    Sample Tested: PC-3 human prostate cancer cell line
    Species: Human
    Verified Customer | Posted 01/10/2018
    Thioredoxin Reductase 1/TRXR1 Antibody (489804) MAB7428

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