Human/Mouse/Rat Thioredoxin Reductase 1/TRXR1 APC‑conjugated Antibody

R&D Systems | Catalog # IC7428A

R&D Systems
Discontinued Product
IC7428A has been discontinued. View all Thioredoxin Reductase 1/TRXR1 products.

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Intracellular Staining by Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 489804
View all Thioredoxin Reductase 1/TRXR1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Thioredoxin Reductase 1/TRXR1
Met1-Ile497
Accession # Q16881

Specificity

Detects mouse Thioredoxin Reductase 1/TRXR1 in direct ELISAs and human, mouse, and rat Thioredoxin Reductase 1/TRXR1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat Thioredoxin Reductase 1/TRXR1 APC‑conjugated Antibody

Detection of Thioredoxin Reductase 1/TRXR1 antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of Thioredoxin Reductase 1/TRXR1 in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse/Rat Thioredoxin Reductase 1/TRXR1 APC-conjugated Monoclonal Antibody (Catalog # IC7428A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse/Rat Thioredoxin Reductase 1/TRXR1 APC‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Thioredoxin Reductase 1/TRXR1

Thioredoxin reductase 1 (TRXR1) is an approximately 70 kDa member of the class-I pyridine nucleotide-disulfide oxidoreductase family.  Human TRXR1 is 649 amino acids (aa) in length. Residues 151-152 constitute a propeptide that is deleted from the mature protein. Splicing variants produce five additional isoforms for human TRXR1. Isoform 2 has a 32 aa substitution for aa 107-138 and a deletion of aa 1-106. Isoform 3 has a deletion of aa 1-51 and a 49 aa substitution for aa 52-100. Isoform 4 is missing aa 1-98 and has a 3 aa substitution for aa 99-101. Isoform 5 has a deletion of aa 1-150. Residues 56-156 make up a glutaredoxin domain, and residues 520-632 constitute a pyridine nucleotide-disulphide oxidoreductase dimerization domain. In addition, there are three phosphotyrosines at positions 161, 163, and 281, and a selenocysteine at position 648. Human TRXR1 shares 74% and 70% aa sequence identity with mouse and rat TRXR1, respectively. Isoform 1 is involved in glutaredoxin activity as well as thioredoxin reductase activity, and it induces actin and tubulin polymerization, which leads to formation of cell membrane protrusions.  Isoform 4 has been shown to enhance the transcriptional activity of the beta receptor only. Finally, isoform 5 mediates cell death induced by a combination of interferon-beta and retinoic acid. Isoform 1 is expressed mostly in the Leydig cells, but also in the ovary, spleen, heart, liver, kidney, and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in the kidney, uterus, testis, ovary, prostate, placenta, and fetal liver.

Alternate Names

GRIM-12, KDRF, TR1, TRXR1, TRXRD1, TXNR, TXNRD1

Entrez Gene IDs

7296 (Human); 50493 (Mouse); 58819 (Rat)

Gene Symbol

TXNRD1

UniProt

Q16881

Additional Thioredoxin Reductase 1/TRXR1 Products

Product Documents for Human/Mouse/Rat Thioredoxin Reductase 1/TRXR1 APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat Thioredoxin Reductase 1/TRXR1 APC‑conjugated Antibody

For research use only

Related Research Areas

Redox Enzymes

Customer Reviews

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat Thioredoxin Reductase 1/TRXR1 APC‑conjugated Antibody and earn rewards!

Have you used Human/Mouse/Rat Thioredoxin Reductase 1/TRXR1 APC‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies