Serum Albumin Antibody

Detection of Human Albumin by Simple Western^TM.

Simple Western lane view shows lysates of iBJ6 human induced pluripotent stem cell line undifferentiated (0) and hepatocyte differentiated for 14 and 20 days, loaded at 0.2 mg/mL. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human Albumin by Simple Western^TM.

Simple Western lane view shows lysates of human serum, loaded at 1:25000. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Serum Albumin by Immunohistochemistry

The fibrillin-1 C1041G mutation impairs retinal arteriole integrity and functionality in adult mice. (A) Retinal arteriole from 4 month old WT (left panels) and Fbn1C1041G/+ (right panels) mice stained for the VSMC marker alpha SMA (red), IB4 (green), albumin (white) and the nucleus marker Hoechst (blue). Note the focal loss of VSMCs around the retinal arteriole (middle panels, red arrowheads) and extravasated albumin (bottom panels) in 4 month old Fbn1C1041G/+ mice. Quantifications of the alpha SMA positive area over the IB4 positive area and albumin area per field are shown, (n = 5 WT and 5 Fbn1C1041G/+ mice). (B) 3D volume reconstruction of the same image of the Fbn1C1041G/+ arteriole displayed in (A) showing focal loss of VSMCs (left panel, red arrowheads) and extravasated albumin (right panel). (C) Retina whole-mounts from 4 month old WT (left panels) and Fbn1C1041G/+ mice (right panels) stained for IB4 (green), the adherens junction marker VE-cadherin (white) and the nucleus marker Hoechst (Ho, blue) (top panel). The single VE-cadherin channel is shown (bottom panels). The red line delineates the contours of a single endothelial cell. Quantifications of the VE-cadherin positive area over the IB4 positive area and the mean EC surface area are shown (n = 30 ECs from 5 WT and 39 ECs from 6 Fbn1C1041G/+ mice). A—arteriole. *** p < 0.001 vs. WT mice (Student’s t tests). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36291539), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Serum Albumin by Immunohistochemistry

Late losartan treatment rescues the functional defects in the retinal arteriole of adult Fbn1C1041G/+ mice. (A) Schematic representation of the protocol of preventive losartan treatment in WT and Fbn1C1041G/+ animals. The treatment was initiated at 3 months for a duration of 6 months. (B) IB4 (green), alpha SMA (red), serum albumin (white), and Col IV (blue) stained retinal arteriole from 9-month-old WT and Fbn1C1041G/+ mice treated or not with losartan. (C) Images of the single IB4 channel (green) from the merged pictures shown in (B). Quantification of the arteriolar diameter, measured at the first bifurcation (red line) is shown (n = 5 H2O-treated WT; 6 H2O-treated Fbn1C1041G/+; 7 losartan-treated WT; 6 losartan-treated Fbn1C1041G/+ mice). D. Images of the single Col IV channel (blue) from the merged pictures shown in (B). Quantification of the Col IV positive area over the IB4 positive area is shown.). (E,F) Same experimental setup as in (D) for alpha SMA (red) in (E) and for albumin (white) in (F). White arrowheads—focal loss of VSMCs. A—arteriole. * p < 0.05; ** p < 0.01; *** p < 0.001 vs. respective WT mice. °° p < 0.01; °°° p < 0.001 vs. respective untreated mice (one-way ANOVA followed by Tukey’s post-test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36291539), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Serum Albumin by Immunocytochemistry/ Immunofluorescence

Hepatocyte-specific GABAAR gamma 2 upregulation in response to obesogenic diets determined by immunofluorescence.A Experimental design. C57BL/6 male mice were fed with RCD or HFD over the course of 6 weeks. B Representative confocal microscopy images and quantification (C) of GABAAR gamma 2 levels in mice fed with RCD or HFD (n = 4 mice per condition). F4/80 and albumin are markers specific for murine macrophages and hepatocytes, respectively. For statistical analyses, p values are calculated by two-tailed unpaired Mann–Whitney U-test. Scale bar: 10 μm. a.u.: arbitrary units. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41419-024-06633-6), licensed under a CC-BY license. Not internally tested by R&D Systems.