Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Knockout Validated, Western Blot, Simple Western, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all STAT1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human STAT1

Specificity

Detects human and mouse STAT1 p91 in Western blots. In Western blots, less than 1% cross-reactivity with human or mouse STAT1 p84 is observed. Additionally, no cross-reactivity with the other STATs has been detected by immunoprecipitation or Western blotting.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse STAT1 p91 Antibody

Detection of Human and Mouse STAT1 p91 antibody by Western Blot.

Detection of Human and Mouse STAT1 p91 by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse STAT1 p91 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST1) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for STAT1 p91 at approximately 91 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunoprecipitation of Human STAT1.

Immunoprecipitation of Human STAT1.

STAT1 p91 was immunoprecipitated from 200 µg of cell lysate of HeLa human cervical epithelial carcinoma cell line following incubation with 3 µg Goat Anti-Human/Mouse STAT1 p91 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST1) overnight at 4 °C. STAT1 p91-antibody complexes were absorbed using Protein G sepharose (Invitrogen, Catalog # 10-1242). Immunoprecipitated STAT1 p91 was detected by Western blot using 1 µg/mL Human STAT1 Monoclonal Antibody (Catalog # MAB14901). View our recommended buffer recipes for immunoprecipitation.
Western Blot Shows Human STAT1 Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human STAT1 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and STAT1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/Mouse STAT1 p91 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST1) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for STAT1 at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot Shows Human STAT1 Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human STAT1 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line, STAT1 knockout (KO) HeLa cell line, STAT2 KO HeLa cell line, STAT3 KO HeLa cell line, STAT5a KO HeLa cell line, STAT5b KO HeLa cell line, and STAT6 KO HeLa cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/Mouse STAT1 p91 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST1) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for STAT1 at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human STAT1 by Simple WesternTM.

Simple Western shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.5 mg/ml. A specific band was detected for STAT1 at approximately 84 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse STAT1 p91 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST1). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Applications for Human/Mouse STAT1 p91 Antibody

Application
Recommended Usage

Immunoprecipitation

3 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line, see our available Western blot detection antibodies

Knockout Validated

STAT1 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in STAT1 knockout HeLa cell line.

Simple Western

10 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line

Western Blot

0.25-1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line

Reviewed Applications

Read 1 review rated 5 using PAF-ST1 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: STAT1

STAT1 is a member of the STAT family of cytoplasmic transcription factors that mediate cytokine, growth factor and hormone receptor signal transduction. STAT1 is associated with type I and II interferon signaling. Phosphorylation of STAT1a at Y701 leads to dimerization and translocation to the nucleus to activate gene transcription. Human STAT1 shows 93% and 94% aa identity with mouse and rat STAT1, respectively, over the region used as an immunogen. This region is identical between isoforms STAT1a (91 kDa) and STAT1b (84 kDa).

Long Name

Signal Transducer and Activator of Transcription 1

Alternate Names

CANDF7, ISGF-3, STAT91

Entrez Gene IDs

6772 (Human); 20846 (Mouse)

Gene Symbol

STAT1

Additional STAT1 Products

Product Documents for Human/Mouse STAT1 p91 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse STAT1 p91 Antibody

For research use only

Citations for Human/Mouse STAT1 p91 Antibody

Customer Reviews for Human/Mouse STAT1 p91 Antibody (1)

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  • Human/Mouse STAT1 p91 Antibody
    Name: Yongkang Yang
    Application: Western Blot
    Sample Tested: human MDAMB-231 whole cell lysate
    Species: Human
    Verified Customer | Posted 11/28/2024
    Western Blot: whole cell lysates from MDA-MB-231 cells were loaded with10 or 50 ug/lane. 10% SDS-PAGE. STAT1 Antibody (PAF-ST1) was used for primary antibody: 1:1000, 4℃, overnight.
    Human/Mouse STAT1 p91 Antibody PAF-ST1

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