Human Mxi1 Alexa Fluor® 488-conjugated Antibody

Catalog #: AF4185G Datasheet / COA / SDS

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AF4185G-100UG

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Human Mxi1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Mxi1 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) MAD1, rhMAD3, and rhMAD4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Mxi1
Ala81-Ser228
Accession # P50539

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Mxi1

Mxi-1 (Max interactor 1) is a 26 kDa member of the bHLH-leucine zipper family, Myc superfamily of transcription factors. It is a widely expressed transcriptional repressor that heterodimerizes with Max and blocks Max-Myc gene activation. Human Mxi-1 is 228 amino acids (aa) in length. It contains a DNA binding motif
(aa 69‑80) and a bHLH domain (aa 81‑120). Multiple splice variants of Mxi-1 appear to exist. They may incorporate an alternate start site at Met37, a 92 aa substitution for the first 26 N-terminal amino acids, or a deletion of the C-terminal 59 or 74 amino acids. Over aa 81‑228, human Mxi-1 is 95% aa identical to mouse Mxi-1.

Long Name

MAX Interacting Protein 1

Entrez Gene IDs

4601 (Human); 17859 (Mouse); 25701 (Rat)

Alternate Names

BHLHC11; Class C basic helix-loop-helix protein 11; MAD2; MAX dimerization protein 2; MAX interacting protein 1; MAX interactor 1bHLHc11MAD2; max-interacting protein 1; Max-related transcription factor; MGC43220; MXD2; MXI; Mxi1; MXI-WR