Human Netrin-G1a Antibody (1010035)

R&D Systems | Catalog # MAB10019

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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1010035
View all Netrin-G1a Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell, HEK293-derived human Netrin-G1a
His29-Gly503
Accession # Q9Y212

Specificity

Detects human Netrin-G1a in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Netrin-G1a Antibody (1010035)

Detection of Netrin-G1a antibody in HEK293 Human Cell Line Transfected with Human Netrin-G1a antibody by Flow Cytometry.

Detection of Netrin‑G1a in HEK293 Human Cell Line Transfected with Human Netrin‑G1a by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with Netrin-G1a (filled histogram) or irrelevant protein (open histogram) was stained with Mouse Anti-Human Netrin-G1a Monoclonal Antibody (Catalog # MAB10019) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Detection of Netrin-G1a antibody in U87-MG Human Cell Line antibody by Flow Cytometry.

Detection of Netrin‑G1a in U87-MG Human Cell Line by Flow Cytometry.

U87-MG human glioblastoma cell line was stained with Mouse Anti-Human Netrin-G1a Monoclonal Antibody (Catalog # MAB10019) or isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Netrin-G1a Antibody (1010035)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human Netrin‑G1a and U87-MG human glioblastoma cell line

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Netrin-G1a

NTNG-1, known as Netrin-G1 or alternatively Laminet-1 (Gene name: NTNG1), is a member of the UNC-6/Netrin family of proteins. Human Netrin-G1 is a 55 kDa protein and shares 100% homology with its crab-eating macaque, 98% homology with its rat homolog, and 97% homology with its mouse homolog. Netrin-G1 is synthesized as a 539 amino acid (aa) precursor with a 28 aa signal sequence, a 251 aa laminin-related region containing an N-terminal laminin globular domain (domain VI) followed by 3 laminin EGF-like repeats, a GPI (glycosylphosphatidylinisotol) anchor (Ser510), and a C-terminal 28 aa propeptide that is removed in mature Netrin-G1 (1, 3, 4). Netrin-G1 interacts with its ligand, Netrin-G ligand 1 (NGL1) to regulate neuron growth and patterning, axonal subdendritic differentiation, and synapse formation throughout development (1, 2, 4, 6). Abnormal expression of Netrin-G1 via SNPs (single nucleotide polymorphisms) have been implicated in pathogenesis for schizophrenia (5). Netrin-G1 has widespread expression only in vertebrates, occurring in olfactory mitral cells, cells of the inferior colliculus (hearing), dorsal thalamus (behavior), and cells of the deep cerebellar nuclei and inferior olive (motion) (3).

References

  1. Seiradake, E. et al. (2011) EMBO J. 30:4479.
  2. Song, Y. et al. (2013) J Cell Sci. 126:4926.
  3. Nakashiba, T. et al. (2000) J Neuroscience. 20:6540.
  4. Matsukawa, H. et al. (2014) J Neuroscience. 34:15779.
  5. Zhu, Y. et al. (2011) J Genet. 90:499.
  6. Nishimura-Akiyoshi, S. et al. (2007) Neuroscience. 104:14801.

Alternate Names

NetrinG1a, NTG1, NTNG1

Entrez Gene IDs

22854 (Human); 80883 (Mouse)

Gene Symbol

NTNG1

UniProt

Q9Y212

Additional Netrin-G1a Products

Product Documents for Human Netrin-G1a Antibody (1010035)

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Product Specific Notices for Human Netrin-G1a Antibody (1010035)

For research use only

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