Human Notch-4 Intracellular Domain Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB3847G Datasheet / COA / SDS

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FAB3847G-100UG

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Human Notch-4 Intracellular Domain Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Notch‑4 Intracellular Domain in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Notch-1 Intracellular Domain, rhNotch-2 Intracellular Domain, rhNotch-3,

Source

Monoclonal Rat IgG2a Clone # 411913

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Notchâ€‘4 Intracellular Domain
Gly1778-Lys2003
Accession # Q99466

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Notch-4

Notch-4 is a 250 kDa member of the NOTCH family of proteins. It is primarily expressed on endothelium. Human Notch-4 is a 1980 amino acid (aa) type I transmembrane (TM) glycoprotein that contains a 1424 aa extracellular domain (ECD) and a 535 aa cytoplasmic region. Notch-4 undergoes proteolytic processing at multiple sites. There is probable cleavage of the ECD by furin in the Golgi between aa 1310‑1410. This creates a mature, potentially disulfide-linked heterodimer at the cell surface. Upon ligand binding, two sequential cleavages occur, one in the ECD after Ala1431, and a second in the TM domain after Lys1466. This creates a soluble cytoplasmic fragment that interacts with RBP-J kappa and activates genes associated with HES-1. Over cytoplasmic aa 1778‑2003, human Notch‑4 is 78% aa identical to mouse Notch-4.

Entrez Gene IDs

4855 (Human); 18132 (Mouse); 406162 (Rat)

Alternate Names

INT3; neurogenic locus notch homolog protein 4; notch 4; Notch homolog 4; NOTCH3; Notch4; Notch-4