Human Notch-4 Intracellular Domain Alexa Fluor® 532-conjugated Antibody

Catalog #: AF3847X Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3847X-100UG

Save 15% on Select RUO Reagents. See Details.

All Notch-4 Antibodies

Product Details

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Notch-4 Intracellular Domain Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Notch-4 Intracellular Domain in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) Notch-1 Intracellular Domain and rhNotch-2 Intracellular Domain is obser

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Notch-4 Intracellular Domain
Gly1778-Lys2003
Accession # Q99466

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Notch-4

Notch-4 is a 250 kDa heterodimeric membrane protein with a C-terminal transmembrane fragment covalently linked to an N-terminal extracellular fragment. It functions as a receptor for transmembrane ligands such as the Jagged and Delta proteins. Upon ligand binding, it undergoes sequential proteolytic processing to release the intracellular domain (NICD), which is translocated to the nucleus where it activates target genes. In the region used as immunogen, human and mouse Notch-4 share 78% amino acid sequence homology.

Entrez Gene IDs

4855 (Human); 18132 (Mouse); 406162 (Rat)

Alternate Names

INT3; neurogenic locus notch homolog protein 4; notch 4; Notch homolog 4; NOTCH3; Notch4; Notch-4