Human OCIL/CLEC2d Alexa Fluor® 750-conjugated Antibody

Catalog #: AF3480S Datasheet / COA / SDS

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AF3480S-100UG

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All OCIL/CLEC2d Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human OCIL/CLEC2d Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human OCIL/CLEC2d in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse (rm) OCIL is observed and less than 1% cross-reactivity with recombinant human (rh) CLEC2B, and rhCLEC2A i

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human OCIL/CLEC2d
Ser57-Val191
Accession # Q9UHP7

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: OCIL/CLEC2d

Human OCIL/CLEC2d, also known as Lectin-Like Transcript-1 (LLT-1), is a type II transmembrane protein belonging to the natural killer (NK) cell receptor group of the C-type lectin superfamily. It is found on hematopoietic cells, osteoblasts, and chondrocytes. By alternative splicing, at least three isoforms exist. Isoform 1 is synthesized as a 191 amino acid (aa) precursor with a C-terminal 132 aa extracellular domain (ECD) that contains the C-type lectin domain. The aa sequence of human OCIL isoform 1 ECD is 49% and 50% identical to the mouse and rat OCIL ECD, respectively. Human OCIL preferentially binds high molecular weight sulfated glycosaminoglycans and is a ligand for the single human NKR-P1A receptor (CD161). Human OCIL blocks osteoclast differentiation. Engagement of OCIL with CD161 on NK cells also inhibits NK cell-mediated cytotoxicity and IFN-gamma secretion.

Long Name

Osteoclast Inhibitory Lectin

Entrez Gene IDs

29121 (Human); 93694 (Mouse)

Alternate Names

CLAXLLT-1; CLEC2D; C-type lectin domain family 2 member D; C-type lectin domain family 2, member D; C-type lectin related f; Lectin-like NK cell receptor; Lectin-like transcript 1; LLT1; LLT1Osteoclast inhibitory lectin; OCIL; OCILmember D