Human Olfactomedin-4/OLFM4 Alexa Fluor® 594-conjugated Antibody

Catalog #: FAB9218T Datasheet / COA / SDS

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FAB9218T-100UG

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Human Olfactomedin-4/OLFM4 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Olfactomedin-4/OLFM4 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG1 Clone # 806305

Purification

Protein A or G purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Olfactomedin-4/OLFM4
Asp21-Gln510
Accession # Q6UX06

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Olfactomedin-4/OLFM4

Olfactomedin-4 (OLFM4), previously called GC-1 (G-CSF-stimulated clone-1), GW112 or hOlfD, is a 64 kDa secreted glycoprotein that is a member of the Olfactomedin/Noelin family of proteins. Mature Olfactomedin-4 is a 490 amino acid (aa) protein that is secreted mainly as multimers. Human Olfactomedin-4 is mainly expressed by gastrointestinal stem cells, developing granulocytes, type M4 acute myelogenous leukemias, and intestinal and prostate cancer cells and is upregulated by NF-kB signaling. It is reported to facilitate cell-cell adhesion by binding to lectins and cadherins, and to be anti-apoptotic. Mature human Olfactomedin-4 shares 71% aa sequence identity with mouse and rat Olfactomedin-4.

Entrez Gene IDs

10562 (Human); 380924 (Mouse); 290409 (Rat)

Alternate Names

Antiapoptotic protein GW112; bA209J19.1; GC1; G-CSF-stimulated clone 1 protein; GW112; GW112hOLfD; hGC-1; KIAA4294; olfactomedin 4; Olfactomedin-4; OlfD; OLFM4; OLM4; UNQ362