Human Olfactomedin-4/OLFM4 Antibody Summary
Asp21-Gln510
Accession # Q6UX06
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Olfactomedin-4/OLFM4 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line and SW480 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Olfactomedin-4/OLFM4 Monoclonal Antibody (Catalog # MAB9218) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Olfactomedin-4/OLFM4 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Olfactomedin-4/OLFM4 in Capan‑1 (Positive) and U937 (Negative). Olfactomedin-4/OLFM4 was detected in immersion fixed Capan‑1 human pancreatic adenocarcinoma cell line (Positive) and absent in U937 human histiocytic lymphoma cell line (Negative) using Mouse Anti-Human Olfactomedin-4/OLFM4 Monoclonal Antibody (Catalog # MAB9218) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Olfactomedin-4/OLFM4
Olfactomedin-4 (OLFM4), previously called GC-1 (G-CSF-stimulated clone-1), GW112 or hOlfD, is a 64 kDa secreted glycoprotein that is a member of the Olfactomedin/Noelin family of proteins. Mature Olfactomedin-4 is a 490 amino acid (aa) protein that is secreted mainly as multimers. Human Olfactomedin-4 is mainly expressed by gastrointestinal stem cells, developing granulocytes, type M4 acute myelogenous leukemias, and intestinal and prostate cancer cells and is upregulated by NF-kB signaling. It is reported to facilitate cell-cell adhesion by binding to lectins and cadherins, and to be anti-apoptotic. Mature human Olfactomedin-4 shares 71% aa sequence identity with mouse and rat Olfactomedin-4.
Product Datasheets
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