Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Avian - Chicken, Transgenic Mouse

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all ONECUT2/OC-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ONECUT2/OC-2
Gln185-Thr326
Accession # O95948

Specificity

Detects human ONECUT2/OC-2 in direct ELISAs.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human ONECUT2/OC‑2 Antibody

ONECUT2/OC-2 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

ONECUT2/OC-2 in HepG2 Human Cell Line.

ONECUT2/OC-2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Human ONECUT2/OC-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6294) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

ONECUT2/OC‑2 in HeLa Human Cell Line.

ONECUT2/OC‑2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human ONECUT2/OC‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6294) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human ONECUT2/OC‑2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line

Reviewed Applications

Read 1 review rated 5 using AF6294 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ONECUT2/OC-2

ONECUT2 (one "cut" domain family member 2; also OC-2 and HNF6 beta ) is a monomeric 54 kDa (predicted) member of the One Cut-domain containing class of homeodomain proteins. It is expressed in embryonic neural crest plus hepatic, pancreatic, and intestinal tissue. In the adult, it is found in hepatocytes and small intestine epithelial cells. ONECUT2 is a transcriptional regulator that is known to impact the expression of Ngn3, OPN and Thbs4. This likely contributes to embryonic cell differentiation and migration. Human ONECUT2 is 504 amino acids (aa) in length. It contains two DNA binding regions, one classified as a CUT domain (aa 331‑410) and another as a homeobox domain (aa 427-481). There is one potential alternative start site at Met20. Over aa 185-326, human ONECUT2 shares 98% aa identity with mouse ONECUT2.

Long Name

One Cut Homeobox 2

Alternate Names

HNF-6B, OC-2, OC2

Entrez Gene IDs

9480 (Human); 225631 (Mouse)

Gene Symbol

ONECUT2

UniProt

O95948 (Human)

Additional ONECUT2/OC-2 Products

Product Documents for Human ONECUT2/OC‑2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ONECUT2/OC‑2 Antibody

For research use only

Citations for Human ONECUT2/OC‑2 Antibody

Customer Reviews for Human ONECUT2/OC‑2 Antibody (1)

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  • Human ONECUT2/OC-2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: OVCAR-3 human ovarian carcinoma cell line
    Species: Human
    Verified Customer | Posted 09/10/2021
    Human ONECUT2/OC‑2 Antibody AF6294

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