Human Park7/DJ-1 Antibody (421015)

R&D Systems | Catalog # MAB3995

R&D Systems

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Knockout Validated, Western Blot, Immunocytochemistry, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 421015
View all Park7/DJ-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Park7
Met1-Asp189
Accession # Q99497

Specificity

Detects endogenous human Park7 in Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human Park7/DJ-1 Antibody (421015)

Detection of Human Park7/DJ‑1 antibody by Western Blot.

Detection of Human Park7/DJ‑1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, LNCaP human prostate cancer cell line, and CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human Park7/DJ-1 Monoclonal Antibody (Catalog # MAB3995) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Park7/DJ-1 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Park7/DJ‑1 antibody by Simple WesternTM.

Detection of Human Park7/DJ‑1 by Simple WesternTM.

Simple Western lane view shows lysates of LNCaP human prostate cancer cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Park7/DJ‑1 at approximately 26 kDa (as indicated) using 25 µg/mL of Rat Anti-Human Park7/DJ‑1 Monoclonal Antibody (Catalog # MAB3995). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western Blot Shows Human Park7/DJ-1 Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human Park7/DJ‑1 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Park7 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Park7/DJ-1 Monoclonal Antibody (Catalog # MAB3995) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Park7/DJ-1 at approximately 23 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Shows Park7/DJ‑1 Specificity Using Knockout Cell Line.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Park7/DJ‑1 knockout HEK293T cell line (KO). Nitrocellulose membrane was probed with Rat Anti-Human Park7/DJ‑1 Monoclonal Antibody (Catalog # MAB3995) followed by HRP-conjugated secondary antibody. A specific band was detected for Park7/DJ‑1 at approximately 20 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. Primary antibody dilution used: 0.5µg. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Park7/DJ‑1 Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

HEK293T human embryonic kidney parental cell line WT and Park7/DJ‑1 HEK293T KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Rat Anti-Human Park7/DJ‑1 Monoclonal Antibody (Catalog # MAB3995) followed by incubation with an Alexa-fluor 555 conjugated secondary antibody (upper panel). DAPI-only counterstained cells shown on a lower panel. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Primary antibody dilution used: 1µg/mL. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Applications for Human Park7/DJ-1 Antibody (421015)

Application
Recommended Usage

Immunocytochemistry

0.5 µg/mL
Sample:
HEK293T human embryonic kidney cell line

Knockout Validated

Park7/DJ‑1 is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in Park7/DJ‑1 knockout HEK293T cell line.

Simple Western

25 µg/mL
Sample: LNCaP human prostate cancer cell line and HeLa human cervical epithelial carcinoma cell line

Western Blot

0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, LNCaP human prostate cancer cell line, and CHP-100 human neuroblastoma cell line

Reviewed Applications

Read 3 reviews rated 3.7 using MAB3995 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Park7/DJ-1

Park7, also known as DJ-1, is a cytoplasmic protein that belongs to the ThiJ/Pfp1/DJ-1 superfamily of highly conserved proteins that function as protein chaperones, catalases, proteases and kinases. Park7 is widely expressed in the brain as well as in peripheral tissues. It exists as a homodimer that can be localized in the cytoplasm, nucleus and mitochondria. Park7 is a redox-sensitive protein that has been ascribed various functions, including that as a redox sensor and antioxidant protein. Mutations in Park7 are associated with a small percentage of hereditary early onset Parkinson's disease.

Long Name

Parkinson Disease 7

Alternate Names

DJ-1, DJ1

Entrez Gene IDs

11315 (Human); 57320 (Mouse); 117287 (Rat)

Gene Symbol

PARK7

UniProt

Q99497

Additional Park7/DJ-1 Products

Product Documents for Human Park7/DJ-1 Antibody (421015)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Park7/DJ-1 Antibody (421015)

For research use only

Customer Reviews for Human Park7/DJ-1 Antibody (421015) (3)

3.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
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3 Stars
33%
2 Stars
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1 Stars
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  • Human Park7/DJ-1 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human Park7/DJ-1 Antibody (421015) MAB3995
  • Human Park7/DJ-1 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/14/2018
  • Human Park7/DJ-1 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/09/2018

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