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Human PDGF-AA ELISA Standard Curve
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Product Details
Citations (9)
Supplemental Products
Reviews (1)

Human PDGF-AA DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human PDGF-AA. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human PDGF-AA ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PDGF-AA

The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).

Long Name:
Platelet-derived Growth Factor AA
Entrez Gene IDs:
5154 (Human); 25266 (Rat)
Alternate Names:

Citations for Human PDGF-AA DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Perioperative escape from dormancy of spontaneous micro-metastases: A role for malignant secretion of IL-6, IL-8, and VEGF, through adrenergic and prostaglandin signaling
    Authors: R Haldar, LS Berger, E Rossenne, A Radin, A Eckerling, E Sandbank, EK Sloan, SW Cole, S Ben-Eliyah
    Brain, Behavior, and Immunity, 2023-01-13;0(0):.
    Species: Xenograft
    Sample Types: Plasma
  2. Knock-out of 5-lipoxygenase in overexpressing tumor cells-consequences on gene expression and cellular function
    Authors: H Weisser, T Göbel, G Melissa Kr, M Krei beta, C Angioni, D Sürün, D Thomas, T Schmid, AK Häfner, AS Kahnt
    Cancer Gene Therapy, 2022-09-16;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Human Primary Astrocytes Differently Respond to Pro- and Anti-Inflammatory Stimuli
    Authors: P Szpakowski, D Ksiazek-Wi, M Turniak-Ku, I Pacan, A Glabinski
    Biomedicines, 2022-07-22;10(8):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Systemic and mucosal immune profiling in asymptomatic and symptomatic SARS-CoV-2-infected individuals reveal unlinked immune signatures
    Authors: S Ravichandr, G Grubbs, J Tang, Y Lee, C Huang, H Golding, S Khurana
    Science Advances, 2021-10-13;7(42):eabi6533.
    Species: Human
    Sample Types: Nasal Lavage Fluid
  5. In Ovarian Cancer Multicellular Spheroids, Platelet Releasate Promotes Growth, Expansion of ALDH+ and CD133+ Cancer Stem Cells, and Protection against the Cytotoxic Effects of Cisplatin, Carboplatin and Paclitaxel
    Authors: N Casagrande, C Borghese, F Agostini, C Durante, M Mazzucato, A Colombatti, D Aldinucci
    International Journal of Molecular Sciences, 2021-03-16;22(6):.
    Species: Human
    Sample Types: Plasma
  6. Rescuing key native traits in cultured dermal papilla cells for human hair regeneration
    Authors: CM Abreu, MT Cerqueira, RP Pirraco, L Gasperini, RL Reis, AP Marques
    Journal of advanced research, 2020-11-04;30(0):103-112.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Oxidative stress-mediated mitochondrial dysfunction facilitates mesenchymal stem cell senescence in ankylosing spondylitis
    Authors: G Ye, Z Xie, H Zeng, P Wang, J Li, G Zheng, S Wang, Q Cao, M Li, W Liu, S Cen, Z Li, Y Wu, Z Ye, H Shen
    Cell Death Dis, 2020-09-17;11(9):775.
    Species: Human
    Sample Types: Serum
  8. Overexpression of Platelet-Derived Growth Factor and Its Receptor Are Correlated with Oral Tumorigenesis and Poor Prognosis in Oral Squamous Cell Carcinoma
    Authors: LH Lin, JS Lin, CC Yang, HW Cheng, KW Chang, CJ Liu
    Int J Mol Sci, 2020-03-29;21(7):.
    Species: Human
    Sample Types: Serum
  9. Induction of nuclear protein-1 by thyroid hormone enhances platelet-derived growth factor A mediated angiogenesis in liver cancer
    Authors: CY Chen, SM Wu, YH Lin, HC Chi, SL Lin, CT Yeh, WY Chuang, KH Lin
    Theranostics, 2019-04-13;9(8):2361-2379.
    Species: Human
    Sample Types: Cell Culture Supernates


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Reviews for Human PDGF-AA DuoSet ELISA

Average Rating: 2 (Based on 1 Review)

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By Anonymous on 11/03/2023
Sample Tested: Plasma,Urine

Measured plasma at 50-fold dilution, but samples were not in range. We did not have a chance to repeat at a lower dilution factor. Measured urine at 2-fold dilution and obtained values in range.