Human Phospho-Lck (Y394) Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB7500V Datasheet / COA / SDS

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FAB7500V-100UG

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Human Phospho-Lck (Y394) Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Lck when phosphorylated at Y394. Due to high sequence homology, this antibody may cross-react with phosphorylated members of the Src family.

Source

Monoclonal Mouse IgG2b Clone # 755103

Purification

Protein A or G purified

Immunogen

Phosphopeptide containing the human Lck Y394 site
Accession # P06239

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Lck

Lck (p56lck) is a 56 kDa cytosolic phosphoprotein within the Src family of non-receptor tyrosine kinases. The 509 amino acid (aa) human Lck contains an SH3 domain (aa 61-121), an SH2 domain (aa 127-224) and a protein kinase domain (aa 251-259). A short (363 aa) isoform contains alternate sequence starting at aa 348, while a 539 aa isoform contains inserted sequence after aa 321. Lck interacts with T cell CD4 and CD8 molecules and plays a pivotal role in regulating T cell activation. It can be overexpressed in cancers and functions as an oncogene. Y394 is the major site for autophosphorylation that enhances kinase activity, while Y505 is the major phosphorylation site for negative regulation.

Long Name

Lymphocyte Protein Tyrosine Kinase

Entrez Gene IDs

3932 (Human); 16818 (Mouse); 313050 (Rat)

Alternate Names

EC 2.7.10; EC 2.7.10.2; Hck-3; Lck; Lcktkr; Leukocyte C-terminal Src kinase; LSK; LSTRA; Lymphocyte cell-specific protein-tyrosine kinase; lymphocyte-specific protein tyrosine kinase; p56(LSTRA) protein-tyrosine kinase; p56lck; p56-LCK; pp58lck; Protein YT16; Proto-oncogene Lck; proto-oncogene tyrosine-protein kinase LCK; T cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; tyrosine-protein kinase Lck; YT16