Human Phospho-Src (Y416) Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB2685X Datasheet / COA / SDS

Recombinant Monoclonal Antibody

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FAB2685X-100UG

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All Src Antibodies

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Human Phospho-Src (Y416) Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Src when phosphorylated at Y416 in direct ELISAs and Western blots.

Source

Monoclonal Rabbit IgG Clone # 1246F

Purification

Protein A or G purified

Immunogen

Phosphopeptide containing the human Src Y416 site

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Src

The Src family of proteins are intracellular tyrosine kinases involved in cell proliferation, differentiation, motility, and survival. Src family activity is regulated by tyrosine phosphorylation at two sites with opposing effects. Autophosphorylation in the activation loop of the kinase domain (Y419 of human Src) upregulates the enzyme, while phosphorylation in the C-terminal tail (Y530 of human Src) downregulates activity.

Long Name

v-src Sarcoma [Schmidt-Ruppin A-2] Viral Oncogene Homolog

Entrez Gene IDs

6714 (Human); 20779 (Mouse); 83805 (Rat); 1491925 (Viral)

Alternate Names

ASV; c-Src; EC 2.7.10; EC 2.7.10.2; pp60c-src; Rous sarcoma; RSVgp4; Src; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)