Human PI 3-Kinase p110 beta Antibody (269020)

R&D Systems | Catalog # MAB2686

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 269020
View all PI 3-Kinase p110 beta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PI 3-Kinase p110 beta
Met152-Val349
Accession # P42338

Specificity

Detects endogenous human PI 3-kinase p110 beta in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human PI 3-Kinase p110 beta Antibody (269020)

Detection of Human PI 3-Kinase p110 beta antibody by Western Blot.

Detection of Human PI 3-Kinase p110 beta by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human PI 3-Kinase p110 beta Monoclonal Antibody (Catalog # MAB2686) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PI 3-Kinase p110 beta at approximately 110 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human PI 3-Kinase p110 beta Antibody (269020)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: A431 human epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line

Reviewed Applications

Read 3 reviews rated 4 using MAB2686 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PI 3-Kinase p110 beta

Class IA phosphoinositide 3-kinases (PI 3-kinases) are a family of p85/p110 heterodimeric lipid kinases that function as signal transducers downstream of growth factor receptors, mainly receptor tyrosine kinases. Mammals express three class IA catalytic subunits: p110 alpha, p110 beta, and p110 delta. Reports suggest that the p110 beta subunit may have special importance in promoting cell proliferation.

Long Name

Protein Tyrosine Phosphatase 1B

Alternate Names

PI 3Kinase p110 beta, PIK3CB

Entrez Gene IDs

5291 (Human)

Gene Symbol

PIK3CB

UniProt

P42338

Additional PI 3-Kinase p110 beta Products

Product Documents for Human PI 3-Kinase p110 beta Antibody (269020)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PI 3-Kinase p110 beta Antibody (269020)

For research use only

Citations for Human PI 3-Kinase p110 beta Antibody (269020)

Customer Reviews for Human PI 3-Kinase p110 beta Antibody (269020) (3)

4 out of 5
3 Customer Ratings
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4 Stars
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3 Stars
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1 Stars
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Showing  1 - 3 的 3 reviews Showing All
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  • Human PI 3-Kinase p110 beta Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human PI 3-Kinase p110 beta Antibody (269020) MAB2686
  • Human PI 3-Kinase p110 beta Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 02/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human PI 3-Kinase p110 beta Antibody (269020) MAB2686
  • Human PI 3-Kinase p110 beta Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/14/2018

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