Human Plexin A1 Antibody (708954)

R&D Systems | Catalog # MAB65361

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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 708954
View all Plexin A1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Plexin A1
Tyr964-Leu1152
Accession # Q9UIW2

Specificity

Detects human Plexin A1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Plexin A1 Antibody (708954)

Detection of Plexin A1 in MDA-MB-231 cells and Daudi cells.

Plexin A1 was detected in immersion fixed MDA‑MB‑231 human breast cancer cell line (Positive) and absent in Daudi human Burkitt's lymphoma cell line (Negative) using Mouse Anti-Human Plexin A1 Monoclonal Antibody (Catalog # MAB65361) at 3 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Plexin A1 Antibody (708954)

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed MDA-MB-231 human breast cancer cell line and Daudi human Burkitt's lymphoma cell line.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Plexin A1

Plexin A1 is an approximately 200 kDa type I transmembrane member of the Plexin family of Semaphorin signal transducers. It consists of a 1218 amino acid (aa) extracellular domain with one Sema domain, a spacer, and four tandem IPT/TIG domains, a 21 aa transmembrane segment, and a 631 aa cytoplasmic domain. Plexin A1 binds the transmembrane Semaphorin 6D directly and binds the Class 3 secreted Semaphorins indirectly via Neuropilin-1 and -2. It mediates cytoskeletal remodeling that results in cell migration and axon repulsion. Within aa 964-1152 (in the IPT/TIG region), human Plexin A1 shares 93% aa sequence identity with mouse and rat Plexin A1.

Alternate Names

NOVP, PLXN1, PLXNA1

Entrez Gene IDs

5361 (Human); 18844 (Mouse)

Gene Symbol

PLXNA1

UniProt

Q9UIW2

Additional Plexin A1 Products

Product Documents for Human Plexin A1 Antibody (708954)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Plexin A1 Antibody (708954)

For research use only

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